medigraphic.com
Órgano Oficial de la Asociación Mexicana de Infectología Pediátrica y la SLIPE.

2007, Number 82

<< Back Next >>

Rev Enfer Infec Pediatr 2007; 20.21 (82)

Identification of Ureaplasma urealyticum in cerebrospinal fluid of newborns infants with suspicion of neuroinfection by polymerase chain reaction (PCR) Phase of standardization

Reyna FJ, Flores MS, Morales MI, García RS, Ortiz IFJ

Language: Spanish
References: 16
Page: 42-45
PDF size: 177.08 Kb.


ABSTRACT

We report the results of the first phase of a study directed to assess the efficacy of polymerase chain reaction (PCR) in the detection of Ureaplasma urealyticum in the cerebrospinal fluid in newborns. In this phase, we standardized the technique in CSF in newborns. We also describe specific conditions for the amplification of the bacterial genome. Fifty DNA extractions from clinical CSF samples were inoculated with U. urealyticum 104 CFU. We made four dilutions 103, 102, 101 y 100. We analyzed this samples using phenolchloroform method of PCR, and we probe some new conditions. The modifications made by us include dilutions of 103, 102, 101, obtaining a 100% rate of detection of DNA. The most important change was in the aligning temperature. This may have implications for a more reliable diagnosis in the early detection of infection in newborns with suspected infection.


REFERENCES

  1. Alfa M, Embee J, Degagne P. Transmission of Ureaplasma urealyticum from mothers to full and preterm infants. Pediatr Infect Dis J 1995;14:341-5.

  2. Bowman E, Garland S, Fan E. Ureaplasma urealyticum respiratory tract colonization and chronic lung disease in infants with very low birth weight. Prenat Neonatal Med 1997;2:42-7.

  3. Cassel G, Waites KB, Watson H, Crouse D, Harasawa R. Ureaplasma urealyticum intrauterine infection: role in prematurity and disease in newborns. Clin Microbiol 1993;6:69-87.

  4. Kong F, Ma Z, James G. Species identification and subtyping of Ureaplasma parvum and Ureaplasma urealyticum using PCR-based assays. J Clin Microbiol 2000;38:1175-9.

  5. Alonso C, Wauters N, Vermeylen D, Muller M, Serruys E. A fatal case of Mycoplasma hominis meningoencephalitis in a full-term newborn. J Clin Microbiol 1997;35:286-7.

  6. Wientzen R. Genital mycoplasmas and the pediatrician. Pediatr Infect Dis J 1990;9:232-5.

  7. Siber G, Alpert S, Smith A, Lin J, McCormack W. Neonatal central nervous system infection due to Mycoplasma hominis. J Pediatr 1977;90:635-7.

  8. Hjelm E, Jonsell G, Linglof T, Mardh P, Moller B, sedin G. Meningitis in a new born infant caused by Mycoplasma hominis. Acta Paediatr Scand 1980;69:415-8.

  9. Lerer RJ, Kalavsky S. Central nervous system disease associated with Mycoplasma pneumoniae infection: report of five cases and review of the literature. Pediatrics 1973;52:658-68.

  10. Lederman MM, Ellner JJ. Presence of antibodies to Mycoplasma pneumoniae in patients with bacterial meningitis. J Infect Dis 1983;148:363-5.

  11. Kleemola M, Kaythy H. Increase in titers of antibodies to Mycoplasma pneumoniae in patients with purulent meningitis. J Infect Dis 1982;146:284-8.

  12. Reyna FJ, Ortiz IFJ, Plazola CNG, Limón RAE. Meningitis bacteriana en recién nacidos. Experiencia en el Instituto Nacional de Perinatología de 1990 a 1999. Bol Med Hosp Infant Mex 2004;61:402-11.

  13. Blanchard A, Hentschel J, Duffy L, Baldus K, Cassell GH. Detection of Ureaplasma urealyticum by polymerase chain reaction urogenital tract of adults, in amniotic fluid, and in respiratory tract of newborns. CID 1993;17(suppl 1):S148-53.

  14. Glass JL, Lefkowitz EJ, Glass JS, Heiner CR, Chen EY, Cassell H. The complete sequence of the mucosal pathogen Ureaplasma urealyticum. Nature 2000;407:757-62.

  15. Narita M, Matsuzono Y, Togashi T, Kajii N. DNA diagnosis of central nervous system infection by Mycoplasma pneumoniae. Pediatrics 1992:90:250-3.

  16. Razin S, Yogev D, Naot Y. Molecular biology and pathogenicity of Mycoplasmas. Microbiol Mol Biol Rev 1998;62:1094-156.




2020     |     www.medigraphic.com