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TIP Revista Especializada en Ciencias Químico-Biológicas

2009, Number 1

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TIP Rev Esp Cienc Quim Biol 2009; 12 (1)

Evidencia Ultraestructural del Nucléolo de Entamoeba Histolytica

Vázquez-Echeverría C, Zavala G, Chávez B, Echeverría-Martínez OM, Vázquez-Nin GH, Segura-Valdez ML, Jiménez-García LF

Language: Spanish
References: 16
Page: 34-37
PDF size: 112.95 Kb.


ABSTRACT

Although cell structure of Entamoeba histolytica is well known, the presence of nucleolar material has not been described with the electron microscope. Here we use light and electron microscopy cytochemical techniques to search for evidence of nucleolar material in this amoeba trophozoyte. Toluidine blue for RNA and silver staining for nucleolar organizer stain peripheral intranuclear material. With the electron microscope, a similar material is fibro-granular and is contrasted with techniques for ribonucleoproteins and nucleolar organizer, but it is negative for DNA. These results show ultrastructural evidence for the presence of a peripheral and ringshaped nucleolus in the nucleus of E. histolytica. It is suggested that this intranuclear organelle is a general feature in eukaryotes.


REFERENCES

  1. Martínez-Palomo, A. Amebiasis (Médica Panamericana, México D.F.,1987).

  2. El-Hashimi,W. & Pittman, F. Ultrastructure of Entamoeba Histolytica trophozoites obtained from the colon and from in vitro cultures. Am. J. Trop. Med. Hyg. 19, 215-226 (1970).

  3. Miller, J.H., Swartzwelder, J.C. & Deas, J.E. A electron microscopic study of Entamoeba histolytica. J. Parasitol.47, 577-587 (1961).

  4. Albach, R.A. Nucleic acids of Entamoeba histolytica. J. Protozool. 36, 197-205 (1989).

  5. Albach, R.A., Booden, T., Boonlayangoor, P. & Downing, S. Entamoeba histolytica: autoradiographic analysis of nuclear sites of RNA synthesis. Exp. Parasitol. 42, 248-259 (1977).

  6. Raska, I., Shaw, P.J. & Cmarko, D. Structure and function of the nucleolus in the spotlight. Curr. Opin. Cell Biol. 18, 325-334 (2006).

  7. Jiménez-García, L.F. et al. Electron microscopy localization of ribosomal DNA in rat liver nucleoli by non-isotopic in situ hybridization. Exp. Cell Res. 207, 220-225 (1993).

  8. Smetana, K. in Methods in Cancer Research (ed. Busch, H.) 361-443 (Academic Press, New York, vol. II, 1967).

  9. Goodpasture, C. & Bloom, S.E. Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53, 37- 50 (1975).

  10. Spector, D.L., Goldman, B.O. & Leinwand, L.A. Cells: a laboratory manual (Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York, vol. II, 1997).

  11. Vázquez Nin, G.H. & Echeverría, O.M. Introducción a la microscopía electrónica aplicada a las ciencias biológicas (Fondo de Cultura Económica, México D.F., 2000).

  12. Bernhard, W. A new staining procedure for electron microscopical cytology. J. Ultrastruct. Res. 27, 250- 265 (1969).

  13. Jiménez-García, L.F. et al. Implications for evolution of nuclear structures of animals, plants, fungi, and protoctists. BioSystems 22, 103-116 (1989).

  14. Jhingan, G.D., Panigrahi, S.K., Bhattacharya, A. & Bhattacharya, S. The nucleolus in Entamoeba histolytica and Entamoeba invadens is located at the nuclear periphery. Mol. Biochem. Parasitol. 167, 72-80 (2009).

  15. Jiménez-García, L.F. et al. Identification of nucleoli in the early branching protist Giardia duodenalis. Int. J. Parasitol. 38, 1297-1304 (2008).

  16. López-Velázquez, G. et al. Electron microscopy analysis of the nucleolus of Trypanosoma cruzi. Microsc. Microanal. 11, 293-299 (2005).




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