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2010, Number 2

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Vet Mex 2010; 41 (2)

Immunogenic evaluation and protection of Pasteurella multocida antigens isolated from clinical cases

Germán Guadarrama CG, León LL, Alonso FMU, Montes de Oca JR, Fernández RP

Language: English/Spanish
References: 33
Page: 101-110
PDF size: 168.27 Kb.


ABSTRACT

The immunogenic protection response to four P. multocida isolations obtained from clinical cases and a reference strain was studied. Isolations were proven as three different immunogens: complete antigen (Ag), washed Ag and culture supernatant. They were subcutaneously administered in SPF light White Leghorn hens. Immune response was evaluated using ELISA test and challenge test evaluated protection response. The ANOVA and Tukey test did not show statistical differences between groups. All isolations using different vaccines induced high protection levels ranging from 87 to 100%. This study indicates that immunization using these three immunogens induce an effective response against P. multocida challenge with the best protection when culture supernatant is used.


REFERENCES

  1. BOYCE JD, ADLER B. The capsule is a virulence determinant in the pathogenesis of Pasteurella multocida M1404 (B:2). Infect Immun 2000; 68:3463-3468.

  2. RHOADES KP, RIMLER RB. Pasteurellosis. In: Calnek BW, Barnes HJ, Bread CW, Reid WR, Yode HW, editors. Diseases of Poultry. 9th ed. Iowa: Iowa State University Press, 1991: 171-204.

  3. CHUNG JY, WILKIE I, BOYCE JD, TOWNSEND KM, FROST AJ, GHODDUSI M et al. Role of capsule in pathogenesis of fowl cholera caused by P. multocida serogroup A. Infect Immun 2001; 69: 2487-2492.

  4. MUTTERS R, IHM P, FREDERIKSEN S, POHL SW, MANNHEIM W. Reclassification of the genus Pasteurella Trevisan 1887 on the basis of deoxyribonucleic acid homology, with proposals for the new species Pasteurella dagmatis, Pasteurella canis, Pasteurella stomatis, Pasteurella anatis and Pasteurella langaa. Int J Syst Bacteriol 1985; 35: 1004-1010.

  5. FEGAN N, BLACKALL PJ, PAHOFF JL. Phenotipic characterization of Pasteurella multocida isolates from Australia poultry. Vet Microbiol 1995; 47: 281-286.

  6. SNIPES KP, HIRD TE, MCCAPES RH. Homogeneity of characteristics of Pasteurella multocida isolated from turkeys and wildlife in California 1985–1988. Avian Dis 1990; 34: 315–320.

  7. MORISHITA TY, LOWENSTINE LJ, HIRSH DC, BROOKS DL. Pasteurella multocida in raptors: prevalence and characterization. Avian Dis 1996 40: 908-918.

  8. DWIGHT CH, YUAN CZ.. Veterinary Microbiology, 2nd ed. Massachusetts USA: Blackwell Publishing 2004.

  9. WILSON J, SCHURR M, LEBLANC C, RAMAMURTHY R, BUCHANAN K, NICKERSON C. Mechanisms of Bacterial Pathogenicity. J Postgrad Med 2002; 78: 216-224.

  10. HARPER M, COX DA, MICHAEL ST. F, WILKIE WI, BOYCE DJ, ADLER B. A heptosyltransferase mutant of Pasteurella multocida produces a truncated lipopolysaccharide structure and is attenuated in virulence. Infect Immun 2004; 72: 3436-3443.

  11. HEDDLESTONE KL, WATKO LP, REBERS PA. Dissociation of a fowl cholera strain of Pasteurella multocida. Avian Dis 1964; 8: 649-657.

  12. SUTHERLAND IW. Surface carbohydrates of the prokariotic cell. New York: Academic Press Inc 1977.

  13. CHENG KJ, COSTERTON JW. Ultraestructure of cell envelopes of bacteria of the bovine rumen. Appl Microbiol 1975; 28: 841-849.

  14. OPHIR T, GUTNICK DL. A role for exopolysaccharides in the protection of microorganisms from dessication. Appl Environ Microbiol 1994; 60: 740-745.

  15. SMITH HE, DAMMAN M, VAN DER VELDE J, WAGENAAR F, WISSELINK HJ, STOCKHOFE-ZURWIEDEN N et al. Identification and characterization of the cps locus of Streptococcus suis serotype 2: the capsule protects against phagocytosis and is an important virulence factor. Infect Immun 1999; 67: 1750-1756.

  16. GLISSON JR, HOFACRE CP, CHRISTENSEN J. Pasteurella and other related bacterial infections. In: SAIF M, editor. Diseases of poultry. 11th ed. Iowa: Iowa State University Press, 2003: 657-676.

  17. BROGDEN KA, REBERS PA. Serologic examination of the Westphal-type lipopolysaccharides of Pasteurella multocida. Am J Vet Res 1978; 39: 1680-1682.

  18. ORGANIZACIÓN INTERNACIONAL DE EPIZOOTIAS. Manual de las Pruebas de Diagnóstico y de las Vacunas para los Animales Terrestres. [Serie en línea] 1989 [Citado] 2008 Julio 5] Disponible en: URL: http://www.oie.int/esp/normes/mmanual/e_summry.htm 2004

  19. BLACKALL PJ, REID GG. Further efficacy studies of inactivated aluminium hydroxide adsorbed vaccines against infectious coryza. Avian Dis 1987; 31: 527-532.

  20. WILKIE I, GRIMES S, O´BOYLE D, FROST A. The virulence and protective efficacy for chickens of Pasteurella multocida administered by different routes. Vet Microbiol 2000; 72: 57-68.

  21. DUBOIS M, GILLES AK, HALMILTON KJ, REBERS AP, SMITH F. Clorimetric method for determination of sugars and related substances. Anal Chem 1956; 28: 350-356.

  22. ALONSO FMU. Niveles de inmunoglobulinas de la clase IgG totales y específicas contra el Antígeno F5+ de Eschericha coli en suero y calostro de ovejas al parto y suero de corderos durante el periodo perinatal (tesis de maestría). Toluca, Estado de México: Facultad de Medicina Veterinaria y Zootecnia. Universidad Autónoma del Estado de México, 1997.

  23. BRIGGS DJ, SKEELES JK. An enzyme-linked immunosorbent assay for detecting antibodies to Pasteurella multocida in chickens. Avian Dis 1983; 28: 208-215.

  24. STEEL DG, TORRIE JH. Bioestadística, principios y procedimientos. 2ª ed. México: McGraw-Hill Interamericana, 1995.

  25. BOYCE JD, CULLEN PA, NGUYEN V, WILKIE I, ADLER B. Analysis of the P. multocida outer membrane subproteome and its response to the in vivo environment of the natural host. Proteomics 2006; 6: 870-880.

  26. UCHIDA C, KIMURA Y, KUBOTA S, SASAKI O. Protective effect of Pasteurella multocida cell-free antigen and toxoid against challenge with toxigenic strains of P. multocida in mice. J Vet Med Sci 2003; 65: 737-740.

  27. JARVINEN L, HOGENESCH H, SUCKOW AM, OWERSOCK TL. Induction of protective immunity in rabbits by coadministration of inactivated Pasteurella multocida toxin and potassium thiocyanate extract. Infect Immun 1998; 66: 3788-3795.

  28. MENA-ROJAS E, VAZQUEZ CRUZ C, VACA PACHECO S, GARCIA GONZÁLEZ O, PEREZ-MARQUEZ VM, PEREZ-MENDEZ A et al. Antigenic secreted proteins from Haemophilus paragallinarum a 110-kDa putative RTX protein. FEMS Microbiol Lett 2003; 232: 83-87.

  29. MUKKUR T. Demostration of cross-protection between P. multocida type A and P. haemolytic serotype 1. Infect Immun 1997; 18: 583-585.

  30. BROWN JL, DAWE DB, DAVIS RW, FOSTER J, SRIVASTAVA K. Fowl cholera immunization in Turkeys. I. Efficacy of various cell fractions of P. multocida as vaccines. Appl Microbiol 1970; 19: 837-841.

  31. MARSHALL SM, ROBINSON AR, JENSEN MM. Use of an enzyme-liked immunosorbent assay to measure antibody response in turkeys against P. multocida. Avian Dis 1981; 25: 964-971.

  32. AVAKIAN A, DICK WT, HENRY CW. Comparison of various antigens and their ability to detect protective antibodies against Pasteurella multocida using enzymelinked immunosorbent assay. Avian Dis 1986; 30: 527-535.

  33. GANFIELD JD, REBERS AP, HEDDLESTON LK. Immunogenic and toxic properties of a purified lipopolysaccharide-protein complex from Pasteurella multocida. Infect Immun 1976; 14: 990-999.




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