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1995, Number 2

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Vet Mex 1995; 26 (2)

Induction of resistance against Toxoplasma gondii in NIH mice treated with concanavalin A

Bautista GCR, Daza FH, lxta RO, Martínez GF

Language: English/Spanish
References: 19
Page: 113-116
PDF size: 330.34 Kb.


ABSTRACT

The effect of Concanavalin A (Con A) inoculation in mice on the induction of resistance against Toxoplasma gondii infection, was evaluated. Forty eight, parasite free, NIH mice (females weighing 22-24 g), were randomly distributed into eight groups (A1, A2, B1, B2, C1, C2, D1 and D2) of six animals each. These animals were treated intraperitoneally (i.p.) as follows: Those in groups A, B and C received 10, 20 and 40 µg of Con A, respectively; while those in groups D received 0.1 ml of phosphate buffered saline solution. All mice were confronted i.p. with 2 LD50 of T. gondii, RH strain. Animals in groups A1, B1, C1 and D1 were exposed to the protozoan two days after the treatment with Con A, while those in groups A2, B2, C2 and D2, seven days later. The percentages of survivors obtained in a period of 15 days after the infection were: 16.66, 16.66, 0, 0, 33.33, 50, 16.66 and 0 for groups A1, B1, C1, D1, A2, B2, C2, and D2, respectively. To corroborate the results observed in the last four groups, the assay was repeated obtaining similar results, except that the percentage of survivors was 33.33 in the group treated with 40 µg of Con A. Data obtained indicates that Con A injected i.p. in NIH mice induces non-specific resistance against T. gondii. The highest protection was obtained applying 20 µg of the mitogen seven days before the exposition to the protozoan.


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