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ISSN 0185-3252 (Print)
Revista de la Asociación Médica del Centro Médico ABC

2009, Number 4

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An Med Asoc Med Hosp ABC 2009; 54 (4)

Comparative study short and long human gametes coincubation of in vitro fertilization

Linder EC, Navarro MC, González CJ, Álvarez VR, Olguín OAA, Shaw DRJ

Language: Spanish
References: 13
Page: 206-210
PDF size: 55.59 Kb.


ABSTRACT

In conventional in vitro fertilization, the oocyte exposure to sperm is 16 to 20 hours in average. Long time exposure could limit the implantation and pregnancy rate. The short gamete coincubation has demonstrated better results on implantation and pregnancy rates. Objectives: To evaluate fertilization, implantation and pregnancy rates. Material and methods: 284 patients between January 2000 and December 2006 were studied. Group A (n = 225), 3212 coincubated oocytes for 2 hours. Group B (n = 59), 654 coincubated oocytes for 16 to 20 hours. Results: The study showed better embryo quality in the short coincubation group on day 3 (p = 0.023). Implantation rate and gestational sac ratio demonstrate a significant difference between groups (p ‹ 0.0001). b-hCG levels were significantly higher in group A (p = 0.001 and p ‹ 0.001 respectively). Pregnancy rate between group A (110/225 = 48.9%) and group B (12/59 = 20.3%) result highly significant (p ‹ 0.0001). Conclusions: The short exposure of gametes increases implantation and pregnancy rates. The embryo quality using short coincubation is significantly higher.


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