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Anales de la Sociedad Mexicana de Oftalmología y Archivos de la Asociación Para Evitar la Ceguera en México

2009, Number 6

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Rev Mex Oftalmol 2009; 83 (6)

Polimorfismo del gen TLR2 como factor de riesgo en la infección oftálmica por adenovirus

Amato-Almanza M, Bautista LVM, Pérez-Cano HJ, Mejía-López H

Language: Spanish
References: 24
Page: 381-384
PDF size: 91.34 Kb.


ABSTRACT

Introduction: Toll-like receptor 2 (TLR-2), plays a central role in the innate immune response to a wide variety of microorganisms. Polymorphisms for TLR-2 gene (Arg677Trp, Arg753Gln) are associated with high risk infections of Mycobacterium sp, Candida albicans, Herpes Simplex Virus Type 2 and others. TLR2 gene polymorphisms are critical to TLR dimerization and signaling.
Objective: Investigate the relationship between TLR2 gene plymorphisms and adenoviral ocular infection in Mexican population.
Methods: Thirty three samples from patients with adenoviral conjunctivitis and ten samples from healthy subjects as controls were studied. DNA genomic was extracted, a region TLR2 gene which encodes the cytosolic part of the receptor was amplified by PCR and analyzed by direct sequencing.
Results: We did not found heterozygous for the TLR2 Arg677Trp and Arg753Gln. However we found Phe707Phe in five patients with a heterozygous frequency of 7.5%, and control samples do not showed any polymorphisms.
Conclusions: The Phe707Phe polymorphism could play an important role in the infection risk by adenovirus, it is important to increase the population to know the real allelic frequency and to determine if this genetic variant is a true marker of susceptibility to infection in the Mexican population.


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