Use of beer as an alternative supplement for factor V in the in vitro growth of Actinobacillus pleuropneumoniae factor V dependent

Susana Patricia Miranda; Gabriela Bárcenas; María Eugenia Rosales; Juan Antonio Montaraz

1994, Number 2

Vet Mex 1994; 25 (2)

Use of beer as an alternative supplement for factor V in the in vitro growth of Actinobacillus pleuropneumoniae factor V dependent

Miranda SP, Bárcenas G, Rosales ME, Montaraz JA

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The present study tested the use of beer as a substitute for factor V in the in vitro growth of Actinobacillus pleuropneumoniae serotypes 1,3,5,7,9,11 and 12 factor V-dependent. A culture kinetics of A. pleuropneumoniae serotype 1 grown on brain and heart infusion broth, supplemented with either 0.01% nicotine-adeninedynucleotide (BHI-NAD) or 20% beer (BHI-C), was compared. Generation time was shorter and adaptation phase larger on BHI-C; however, at the end of the culture, equivalent quantities of microorganisms were obtained in both media. During the kinetics, excretion of haemolysin was evaluated in both media, and it was observed that beer did not inhibit synthesis and excretion of this important toxin. It is concluded that beer is an economic and easy to handle alternative for factor V in the culture of A. pleuropneumoniae.

REFERENCES
Frey, J. and Nicolet, J.: Regulation of hemolysin expression in Actinobacillus pleuropneumoniae serotype 1 by Ca²⁺Immunol., 56: 2570-2575 (1988).
Frey, J. and Nicolet, J.: Hemolysin patterns of Actinobacillus pleuropneumoniae. J. Clin. Microbiol., 28: 232-236 (1990).
Gunnarsson, A., Hurvell, B. and Biberstein, E.L.: Serologic studies of porcine strains of Haemophilus parahaemolyticus (pleuropneumoniae): Antigenic specificity and relationship bet-ween serotypes. Am. J. vet. Res., 33: 1286-1292 (1976).
Inzana, T,J., Ma, J., Workman, T., Gogolewski, R.P. and Anderson, P.: Virulence properties and protective efficacy of the capsular polymer of Haemophilus (Actinobacillus) pleuropneumoniae serotype 5. Infect. Immunol., 56: 1880-1889 (1988).
Kamp, E.M., Popma, J.K., Anakotta, J. and Smits, M.A.: Identification of the hemolytic and cytotoxic proteins of Actinobacillus pleuropneumoniae by use of monoclonal antibodies. Infect. Immunol., 59: 3079-3085 (1991).
Maudsley, J.R. and Kadis, S.: Growth and hemolysin production by Haemophilus pleuropneumoniae cultivated in a chemical defined medium. Can. J. Microbiol., 32: 801-805 (1986).
Moller, K. and Kilian, M.: V factor-dependent members of the amily Pasteurellaceae in the porcine upper respiratory tract. J. Clin. Microbiol., 28: 2711-2716 (1990).
Niven, D.F. and Reilly, T.O.: Significance of V-factor dependency in the taxonomy of Haemophilus species and related organism. Int. J. Syst. Bacteriol., 40: 1-4 (1990).
Pohl, S., Bertschinger, H.U., Frederiksen, W. and Mannheim, W.: Transfer of Haemophilus pleuropneumoniae and the Pasteurella haemolytica-like organism causing porcine necrotic pleuropneumonia to the genus Actinobacillus (Actinobacillus pleuropneumoniae) on the basis of phenotypic and DNA relatedness. Int. J. Syst. Bacteriol., 33: 510-514 (1983).
Shope, R.E., White, D.C. and Leidy, G.: Porcine contagious pleuropneumonia. I. Experimental transmission, etiology and pathology. J. exp. Med., 119: 357-368 (1964).