Histochemical characteritics of intestinal mucosae in female rat, during their first year of age

Leticia Ramírez Martínez; Angélica González Maciel; Joaquín Cravioto

1994, Number 1

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Vet Mex 1994; 25 (1)

Histochemical characteritics of intestinal mucosae in female rat, during their first year of age

Ramírez ML, González MA, Cravioto J

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Language: English/Spanish
References: 22
Page: 37-40
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ABSTRACT

The small and large intestine of 69 Wistar rats, aged 1 of 360 days, were studied in order to examine the histochemical characteristics of the intestinal mucins of female rats as well as their distribution under normal conditions. Tissue samples (2 cm) were collected from the duodenum, jejunum, ileum, colon and rectum. They were processed and included in paraffin for microscopic view (5 m thick transversally cut samples). The staining techniques used were Schiff periodic acid, Alcian blue (pH 2.5) and fuchsin-aldehyde in order to determine the presence of mucins. Results showed that the mucins from the villi and crypts from the small intestine are acidie, neutral and a mixture of both the firts 28 days of life. At later ages, up to 360 days, the neutral mucins are limited to intestinal crypts. In the large intestine, in the ages studied, weakly sulfated acidic mucins were found at the base of the crypts, while a mixture of mucin and occasionally some neutral ones were found in the apical area of the crypt. In the first stage of development of the female rat, neutral mucins seem to predominate while in the adult rat, acidic mucins dominate. This is similar to what is seen in the male rat during the same age periods, which demonstrates that female hormonal cycles have no influence on the histochemical characteristics of the intestine.

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