1994, Number 1
<< Back Next >>
Vet Mex 1994; 25 (1)
Number and pattern distribution of Plasmocytes in the cervix uteri of gills under both follicular and luteal phases
Lavielle RE, Pérez MM, Hernández GR, Martínez MJJ
Language: English/Spanish
References: 20
Page: 29-31
PDF size: 335.08 Kb.
ABSTRACT
The objetive of this study was to find out the number and pattern distribution of plasmocytes (P) present in the
cervix uteri (CU) of gilts under different hormonal conditions. Gilts in the follicular and luteal phases were sampled in a slaughterhouse in order to perform quantitative and distribution studies. Two cm
3 samples from cranial, medium and caudal regions of the CU were taken and immersed in saline-formaline solution during 24 hours. Thereafter, they were processed and included in paraffin wax and cut in thin 6 µm sections and stained with Giemsa. The slides were observed with a light microscope under the 40X objetive. The total number of P were counted in 16 fields selected at random to determine the number of P per mm
2. Statistical analysis showed significant differences (P ‹ 0.05), when anatomical regions and hormonal conditions were compared. These results suggest that the immune secretory system of cranial and middle parts of the CU in the gilt shows different ability for local production of antibodies due to differences both in pattern distribution of P within anatomical regions and in the response to hormone conditions.
REFERENCES
Ansar-Ahmed, S., Penhale, W.J. and Talal, N.: Sex hormones, immune responses and autoimmune diseases. Am. J. Pathol., 121: 531-551 (1985).
Banks, J.W.: Applied Veterinary Histology. Williams & Wilkins, Baltimore, Maryland, 1974.
Estrada, F.E., Peralta, Z.L. y Rivas, M.P.: Manual de Técnicas Histológicas. A.G.T., México, D.F., 1982.
García, T.F. y Ocampo, L.A.: Interacciones entre los sistemas inmunitario y gonadal. Ciencia, 42: 155-169 (1991).
Lander-Chacin, M.F., Hansen, P.J. and Drost, M.: Effects of stage of the oestrus cycle and steriod treatment of uterine immunoglobulin contentand polymorphonuclear leukocytesin cattle. Theriogenology, 34: 1169-1184 (1990).
Lozano, C.B. y Tolosa, S.J.: Cuantificación de células cebedas y de eosinófilos en la mucosa del cuello uterino de ganado bovino Cebú en diferentes etapas reproductivas. Vet. Méx., 20: 393-396 (1989).
Lynch, M., Raphael, S., Mellor, L., Spare, D. e Inwood, M.: Métodos de Laboratorio, Interamericana, México, D.F., 1977.
Márques de, C.: Probabilidad y Estadística. McGraw-Hill, México, D.F., 1990.
Mitchell, G., Liu, I., Perryman, L., Stabenfeldt, G. and Hughes, J.P.: Preferential production and secretion of immunoglobulins by the equine endometrium-a mucosal system. J. Reprod. Fert., 32(Suppl) 161-168 (1982).
Murdoch, A., Buckley, C.H. and Fox, H.: Hormonal control of the secretory immune system of the human uterine cervix. J. Reprod. Immunol., 4: 23-30 (1982).
Sims, M. and Eiler, H.: Porcine mastitis-metritis-agalactia (MMA) syndrome: Mammary gland responsiveness to oxytocin given to healthy sows during lactation. Am. J. vet. Res., 40: 1104-1106 (1979).
Steel, D. and Torrie, H.: Biestadística: Principios y Procedimientos. McGraw-Hill, México, D.F., 1989.
Sunyol, O.M. y Doporto, J.: Tratamiento profiláctico y terapéutico del síndrome metritis, mastitis y agaláctia del porcino. Vet. Méx., 5: 119-124 (1974).
Tabibzadeh, S. and Satyaswaroop, P.: Sex steroid receptors in lymphoid cells of human endometrium. Am. J. Clin. Pathol., 91: 656-663 (1989).
Valencia, M.J.: Fisiología de la Reproducción Porcina. Trillas, México, D.F., 1986.
Waelchli, R.O. and Winder, N.C.: Immunohistochemical evaluation of the equine endometrium during the oestrus cycle. Equine vet. J., 19: 299-302 (1987).
Watson, E.D. and Stokes, C.R.: Plasma cell numbers in uterine of mares with persistent endometritis and in ovariectomised mares treated with ovarian steroids. Equine vet. j., 20: 424-425 (1988).
Wielen van der, A.L. and King, G.J.: Intraepithelial lymphocytes in the bovine uterus during the oestrus cycle and early gestation. J. Reprod. Fert., 70: 457-462 (1984).
Wira, C.R. and Sandoe, C.P.: Sex steroid hormone regulation of IgA and IgG in rat uterine secretions. Nature, 268: 534-536 (1977).
Zapata, A.G. and Cooper, E.L.: The Immune System: Comparative Histophysiology. John Wiley and Sons, London, 1990.