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Coordinación General de Investigación de la Facultad de Salud Pública y Nutrición y la Dirección General de Sistemas e Informática de la Universidad Autónoma de Nuevo León

2009, Number 1

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Rev Salud Publica Nutr 2009; 10 (1)

Efecto antagónico de lactobacillus plantarum aislado de pastizal de finca lechera

Alvarado- Rivas CC, Díaz- Rivero CG

Language: Spanish
References: 36
Page: 35
PDF size: 111.34 Kb.


ABSTRACT

The antagonism of Lactobacillus plantarum against undesiderable bacteria has important applications in food biopreservation and in probiotic therapy. The antagonistic capacity of six strains of L. plantarum isolated from grass of milking farm which were labeled L. plantarum 1, 4, 20, 37, 50, 58 and tested against Salmonella enteritidis CVCM 446, Salmonella enteritidis isolated from a food outbreak in Mérida- Venezuela, Salmonella typhi isolated from raw milk collected in a local farm and Listeria monocytogenes CVCM 497. The well-agar diffusion method was used with the supernatant of each lactobacilli strain neutralized with NaOH 1N, treatments with peroxidase (32 μg/ml) and with tripsin (2 mg/ml) and heating at 120ºC for 20 minutes, with their respective subsequent controls of residual antagonistic activity. L. plantarum 1, 4, 20, 37, 50 and 58 showed antagonic activity (AA) against S. typhi and L. monocytogenes with inhibition zone of 9-11 mm due to the presence of organic acids. The chemical nature of the supernatant of L. plantarum 37 active against Salmonella typhi could not be established, whereas the AA of L. plantarum 58 against L. monocytogenes which showed inhibition zone of 18 mm was lost with the tripsin treatment but was not affected by heat treatment indicating the presence of heat stable molecule (s) of protein nature. The strains tested showed antagonistic activity that may require further studies, particularly L. plantarum 58 for its interesting action against Listeria monocytogenes and strain 37 for its antagonism against S. typhi.


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