2009, Number 5
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Rev Mex Oftalmol 2009; 83 (5)
Hallazgos clínicos e inmunológicos en pacientes con conjuntivitis alérgica crónica
Ibáñez-Valderrama G, Santacruz-Valdés C, Aguilar-Velázquez G, Linares M, Jiménez-Martínez MC
Language: Spanish
References: 12
Page: 296-300
PDF size: 191.94 Kb.
ABSTRACT
Objective: To determine CD30 expression on CD8+ T cells and cytokine production before and after antigenic stimulation
in vitro. Patients, materials and methods: We included 12 patients with clinical diagnosis of vernal keratoconjunctivitis, divided into two groups according to skin test reaction. Cytometric analisis was performed in mononuclear cells to determine de frequency of CD8, CD30, IL-4 and IL-5 before and after
in vitro antigenic stimulation. Results were analyzed with Sigma Stat software and significant differences were considered when p‹0.05. Results: Ocular findings in conjunctivitis allergic patients were objectively evaluated by developing a visual analogue scale. Frequency of CD8+CD30+ T cells was statistically increased in both groups after
in vitro stimulation. CD8+CD30+ T cells contributed significantly to the Th2 microenvironment with high frequency of IL-4+ and IL-5+ cells (Tc2 cells) than CD8+CD30- T cells. Conjunctivitis allergic patients whit skin reactivity produced after
in vitro stimulation more IL-4 than patients without cutaneous reactivity (p=0.019). Conclusions: This study allows linking the expression of CD30 on CD8+ T cells. We suggest that patients with clinical ocular similarities have immunological differences showed
in vivo with the skin reactivity to allergens and,
in vitro, with increased frequency CD8+CD30+IL-4+ T cells.
REFERENCES
Phipatanakul W. Allergic rhinoconjunctivitis: epidemiology.Immunol Allergy Clin North Am 2005; 25(2):263-281, vi.
Pantoja-Melendez C. Reporte principales causas de consulta Instituto de Oftalmología Fundación Conde de Valenciana 2006.
Maggi E, Biswas P, Del Prete G, Parronchi P, Macchia D, Simonelli C, Emmi L, De Carli M, Tiri A, Ricci M, et al. Accumulation of Th-2-like helper T cells in the conjunctiva of patients with vernal conjunctivitis. J Immunol 1991; 146(4):1169- 1174.
Rojas-Ramos E, Garfias Y, Jimenez-Martinez MC, Martinez- Jimenez N, Zenteno E, Gorocica P, Lascurain R. Increased expression of CD30 and CD57 molecules on CD4+ T cells from children with atopic asthma: A preliminary report. Allergy Asthma Proc 2007; 28(6):659-666.
Garfias Y, Ortiz B, Hernández J, Magaña D, Becerril M, Zenteno E, Lascurain R. CD4+CD30+ T cells perpetuate IL-5 production in Dermatophagoides pteronyssinus allergic patients. Allergy 2006; 61:27-34.
Machura E, Mazur B, Pieniazek W, Karczewska K. Expression of naive/memory (CD45RA/CD45RO) markers by peripheral blood CD4+ and CD8 + T cells in children with asthma. Arch Immunol Ther Exp (Warsz) 2008; 56(1):55-62.
Meyer EH, DeKruyff RH, Umetsu DT. T cells and NKT cells in the pathogenesis of asthma. Annu Rev Med 2008; 59:281- 292.
Poulsen LK, Hummelshoj L. Triggers of IgE class switching and allergy development. Ann Med 2007; 39(6):440-456.
Leonardi A, DeFranchis G, Zancanaro F, Crivellari G, De Paoli M, Plebani M, Secchi AG. Identification of local Th2 and Th0 lymphocytes in vernal conjunctivitis by cytokine flow cytometry.Invest Ophthalmol Vis Sci 1999; 40(12):3036-3040.
Gemou-Engesaeth V, Fagerhol MK, Toda M, Hamid Q, Halvorsen S, Groegaard JB, Corrigan CJ. Expression of activation markers and cytokine mRNA by peripheral blood CD4 and CD8 T cells in atopic and nonatopic childhood asthma: effect of inhaled glucocorticoid therapy. Pediatrics 2002; 109(2):E24.
Stanciu LA, Roberts K, Papadopoulos NG, Cho SH, Holgate ST, Coyle AJ, Johnston SL.IL-4 increases type 2, but not type 1, cytokine production in CD8+ T cells from mild atopic asthmatics. Respir Res 2005; 6:67.
Leonardi A.Vernal keratoconjunctivitis: pathogenesis and treatment. Prog Retin Eye Res 2002; 21(3):319-339.