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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

1999, Number 4

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Rev Biomed 1999; 10 (4)

Blastocystis hominis infection in hospitalized pediatric patients

Requena-Certad I, Devera R, Agreda Y, Córdova Y, Castillo H, Velásquez V

Language: Spanish
References: 45
Page: 199-208
PDF size: 44.00 Kb.


ABSTRACT

Introduction. Blastocystis hominis is a polymorphic protozoan that causes blastocystosis, a recently recognized intestinal parasitosis, however many controversial points and unknown factors concerning B. hominis still exist. Blastocystosis has started to be studied with greater interest in the pediatric population, but available information is still limited in Venezuela.
Objective. To determine the prevalence and clinical relevance of B. hominis infection in a sample of children admitted to the “Ruíz y Páez” University Hospital in Ciudad Bolívar, Venezuela.
Material and methods. A fecal parasitology study was carried out from January to May 1998 on 115 children of both sexes aged under 12 years old and admitted to the pediatric services of the “Ruíz y Páez” University Hospital. A fecal sample obtained by spontaneous evacuation was analyzed by direct examination in 0.85% saline solution and lugol and by the concentration methods of Faust and Willis. The children parasitized with B. hominis were submitted to clinical evaluation.
Results. Infection with some parasite and/or commensal was present in 62.6% of the sample (72/115). The prevalence of B. hominis was 13% (15/115). The infection did not show age (χ2= 6,96 f.g. = 5) or sex (p › 0.05) preference. The protozoan was present in association with other microorganisms in 53.3% of cases and as the only parasite in 46.7%. Giardia lamblia was the parasite most frequently present in association. Symptoms were present in 66.7% of cases and absent in 33.3%. Fatigue, abdominal pain and headache were the most frequent clinical findings.
Conclusion. The prevalence of B. hominis infection in the group under study was relatively high, however, further studies using control groups are necessary to determine the actual clinical importance of this controversial intestinal parasite.


REFERENCES

  1. Zierdt CH, Rude WS, Bull BS. Protozoan characteristics of Blastocystis hominis. Am J Clin Pathol 1967; 48:495-501.

  2. Sheehan DJ, Raucher BG, Mckitrick JC. Association of Blastocystis hominis with signs and symptoms of human disease. J Clin Microbiol 1986; 24:548-50.

  3. Zierdt CH. Blastocystis hominis-Past and future. Clin Microbiol Rev 1991; 4:61-79.

  4. Boreham PFL, Stenzel DJ. Blastocystis in humans and animals: Morphology, Biology, and Epizootology. Adv Parasitol 1993; 32: 1-70.

  5. Zierdt CH. Blastocystis hominis, a longmisunderstood intestinal parasite. Parasitol Today 1988; 4:15-7.

  6. Silberman JD, Sogin ML, Leipe DD, Graham-Clark C. Human parasite finds taxonomic home. Nature 1996; 380:398.

  7. Stenzel DJ, Boreham PFL. A cyst-like stage of Blastocystis hominis. Inter J Parasitol 1991; 21: 613-5.

  8. Pérez de Suárez E, Guzmán de Rondón C. La morfología del Blastocystis hominis en las heces y evaluación de métodos parasitológicos. GEN 1994; 226-31.

  9. Devera RA, Niebla-Punos G, Nastasi JA, Velásquez VJ., González-Menéses R. Prevalencia de infección por Blastocystis hominis en niños del Estado Bolívar: valor del examen directo de heces en el diagnostico. En: Memorias de las XIV Jornadas Científicas, Tecnológicas y Educativas de Guayana; Noviembre 12-14; Ciudad Bolívar, Venezuela; 1998. p. 28-9.

  10. Pires de Mato C, .Amato Neto V, Braz LMA, Carignani FL, Villela MSH, Pinto THL, et al. Bastocystis hominis. Diagnóstico por exame direto e por coloração com tionina. Rev Soc Bras Med Trop 1998; 31 (sup1):188.

  11. Zaman V. The diagnosis of Blastocystis hominis. J Infec 1996; 33: 15-6.

  12. Torres P, Miranda JC, Flores L, Riquelme J, Franjona R, Perez J, et al. Blastocistosis y otras infeccioes por protozoos intestinales en comunidades humanas ribereñas de la cuenca del Río Valdivia, Chile. Rev Inst Med Trop São Paulo 1992; 34:557-64.

  13. Phillips BP, Zierdt CH. Blastocystis hominis: pathogenic potencial in human patiens and in gnotobiotes. Exp Parasitol 1976; 39:358-64.

  14. McClure HM, Strobert EA, Healy GR. Blastocystis hominis in a Pig-tailed Macaque: a potential enteric pathogen for nohuman primates. Lab Anim Sci 1980; 30:890-4.

  15. Moe KT, Singh M, Howe J, Ho LC, Tan SW, Chen XQ, et al. Experimental Blastocystis hominis infection in laboratory mice. Parasitol Res 1997; 83:319-25.

  16. Walderich B, Bernauer S, Renner M, Knobloch J, Buchard GD. Cytopathic effects of Blastocystis hominis on Chinese hamster ovary (CHO) and adeno carcinoma HT29 cell cultures. Trop Med Inter Health 1998; 3:385-90.

  17. Mercado R, Arias B. Blastocystis hominis: Frecuencia de infección en pacientes ambulatorios del sector norte de Santiago, Chile. Bol Chil Parasitol 1991; 46: 30-2.

  18. Ponce de León P, Svetaz MJ, Zdero M. Importancia del diagnóstico de Blastocystis hominis en el examen parasitológico de heces. Rev Latamer Microbiol 1991; 33:159-64.

  19. Guimaraes S, Sogayar MIL. Blastocystis hominis: Occurrence in children and staff members of municipal day-care centers from Botucatu, São Paulo State, Brazil. Mem Inst Oswaldo Cruz 1993; 88:427-9.

  20. Castrillo de Tirado A, Gonzalez MAJ, Tirado EE. Frecuencia de infección por Blastocystis hominis: un año de estudio. GEN 1990; 44:217-20.

  21. O’Gorman MA, Orenstein SR, Proujansky R, Wadowsky R, Putnam PE, Kocoshis SA. Prevalence and characteristics of Blastocystis hominis infection in children. Clin Pediatric 1993; 32:91-6.

  22. Nimri L. Evidence of an epidemic of Blastocystis hominis infections in preschool children in northern Jordan. J Clin Microbiol 1993; 31: 2706-8.

  23. Kukoschke Kg, Muller HE. Varying incidence of Blastocystis hominis in cultures from faeces of patients with diarrhoea and from healthy persons. Zbl Bakt 1992; 277: 112-8.

  24. Udkow MP, Markell EK. Blastocystis hominis: prevalence in asymptomatic versus symptomatic hosts. J Infec Dis 1993; 168:242-4.

  25. Shlim DR, Hoge CW, Rajah R, Rabold JG, Echeverria P. Is Blastocystis hominis a cause of diarrhea in travelers? Aprospective controlled study in Nepal. Clin Infec Dis 1995; 21: 97-101.

  26. Reinthaler FF, Mascher F, Klem G, Sixl W. A survey of gastrointestinal parsites in Ogun State, southwest Nigeria. Ann Trop Med Parasitol 1988; 82: 181-4.

  27. Jelinek T, Peyerl G, Loscher T, von Sonnenburg F, Nothdurft HD. The role of Blastocystis hominis as a potential intestinal pathogen in travellers. J Infec 1997; 35: 63-6.

  28. Devera R. Blastocystis hominis: o enigma continua. Rev Soc Bras Med Trop 1998; 31: 491-2.

  29. Zaki MAA, Daoud AS, Pugh RNH, Al-Ali F, Al-Mutairi G, Al-Saleh Q. Clinical report of Blastocystis hominis infection in children. J Trop Med Hyg 1991; 94:118-22.

  30. Martín-Sanchez AM, Canut-Blasco A, Rodríguez- Hernández J. Montes-Martínez I, García-Rodriguez JA. Epidemiology and clinical significance of Blastocystis hominis in different population groups in Salamanca (Spain). Eur J Epidemiol 1992; 8:553-9.

  31. Sinniah B, Rajeswari B. Blastocystis hominis infection, a cause of human diarrhea. Southeast Asian. J Trop Med Public Health 1994; 25: 490-3.

  32. Beauchamp S, Flores T, Tarazona S. Blastocystis hominis: prevalencia en alumnos de una escuela básica. Maracaibo, Edo. Zulia, Venezuela. Kasmera 1995; 23: 43-67.

  33. Lozada M, López V, Pacheco M, Bermúdez S, Ladera E. Blastocystis hominis en la población escolar de la Escuela Básica Nacional “Los Magallanes”. Barrio Los Magallanes, Municipio San Diego. Estado Carabobo. Acta Cient Venezol 1995; 46 (suppl 1): 162-3.

  34. Devera RA, Niebla-Punos G, Velásquez VJ, Nastasi JA, González-Meneses R. Prevalencia de infección por Blastocystis hominis en escolares de Ciudad Bolívar, Venezuela. Bol Chil Parasitol 1997; 52:77-81.

  35. Devera R, Velásquez V, Vásquez, M. Blastocistosis en pre-escolares de Ciudad Bolívar, Venezuela. Cad Saude Publica 1998; 18: 401-7.

  36. Melvin DM, Brooke M.M. Métodos de laboratorio para diagnóstico de parasitosis intestinales. 1a ed. México: Nueva Editorial Interamericana, S.A.; 1971. p. 198.

  37. Morales G, Pino LA. Parasitología cuantitativa. 1a ed. Caracas: Fondo Editorial Acta Cientifica Venezolana; 1987. p 132.

  38. Azacón B, Jiménez M. Frecuencia de Blastocystis hominis en pacientes hospitalizados. Hospital Universitario ¨Ruíz y Páez¨. Ciudad Bolívar. 1996-1997. Tesis de Grado. Universidad de Oriente, Estado Bolívar, Venezuela 1997:52.

  39. Biolley MA, Oberg C. Blastocistosis en pacientes asintomáticos en el Hospital Regional de Temuco, Chile. Bol Chil Parasitol 1993; 48: 25-7.

  40. Devera R. Ausencia de Entamoeba histolytica/Entamoeba dispar en Ciudad Bolívar, estado Bolívar, Venezuela. Rev Biomed 1998; 9:199-201.

  41. Mercado R, Guarda ME, Guerra H, Lobos M, Ferrada C, Aias B, et al. Protozoos y helmintos intestinales: frecuencia de infección, estado nutricional y manifestaciones digestivas en niños de hogares de menores y una sala de cuna de Santiago, Chile. Bol Chil Parasitol 1988; 43: 41-6.

  42. Michelli VE. Prevalencia de Blastocystis hominis en pacientes con y sin síntomas gastrointestinales del Hospital “Pedro Rafael Figallo”, Río Caribe, Estado Sucre. Tesis de grado. Universidad de Oriente, Cumaná, Venezuela; 1995. p. 64.

  43. Beltrán M. Blastocystis hominis en los hospitales de Lima. En: Memorias del IX Congreso Latinoamericano de Parasitología; Noviembre 12-18; Caracas, Venezuela; 1989. p. 218.

  44. Waghorn DJ, Hancock P. Clinical significance of Blastocystis hominis. Lancet 1991; 337:609.

  45. Doyle PW, Helgason MM, Mathias RG, Proctor EM. Epidemiology and pathogenicity of Blastocystis hominis. J Clin Microbiol 1990; 28:116-21.




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