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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

1999, Number 3

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Rev Biomed 1999; 10 (3)

Poliarthritis in growing pigs: isolation and characterisation of Mycoplasma hyosynoviae and Acholeplasma granularum

Copes J, Acosta LS, Pellicer K, Stanchi NO, Perfumo JC

Language: Spanish
References: 22
Page: 159-165
PDF size: 187.86 Kb.


ABSTRACT

Objective. To detect the microorganisms belonging to the genus Mycoplasma from joint exudate from growing pigs with polyarthritis lesions.
Materials and Methods. The samples were streaked on modified Hayflick medium and were placed in aerobic, microaerobic, and anaerobic atmosphere. In those cultures in which it was possible to observe growth, they were clonated and streaked on serum free medium. The following biochemical test was performed on the isolate strains: glucose fermentation, arginine hydrolysis, tetrazolium reduction, fosfatase production and film and spot observation. The serotyping of the isolated strains was performed by means of growth inhibition test and indirect epi-immunofluorescence test with reference serum.The protein profiles were compared by means of sodium dodecil sulfate polyacrylamide gel electrophoresis between isolated strains and reference strains Mycoplasma hyosynoviae S16 and Acholeplasma granularum BTS39.
Results. On the basis of the obtained results, two isolated strains correspond to Mycoplasma hyosynoviae and the another strain to Acholeplasma granularum.
Discussion. With this methodology we isolate of M. hyosynoviae and A. granularum from pigs with arthritis for the firts time in Argentina. We conclude that it is of great value for Veterinarians and farmers and that they should take this pathological entity into account in pig production.


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