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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

1999, Number 3

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Rev Biomed 1999; 10 (3)

Comparative study of urease tests for Helicobacter pilory detection in gastric biopsies

Quintana-Guzmán EM, Schosinsky-Nevermann K, Arias-Echandi ML, Davidovich-Rose H

Language: English
References: 43
Page: 145-151
PDF size: 33.08 Kb.


ABSTRACT

Introduction. A positive urease test is strong evidence of Helicobacter pylori (H. pylori) infection. This test is widely used as standard procedure for the detection of this bacteria, because it is a simple, reliable and inexpensive test, and provides quick results.
Material and Methods. Ninety-nine biopsies from patients of the Endoscopy Department at San Vicente de Paul Hospital, Heredia, Costa Rica were studied. Diagnosis of H. pylori infection was based on histological examination and apposition smear stained with Giemsa of biopsies samples from gastric antrum. A comparative study of urease tests for the diagnosis of H. pylori in gastric biopsies was made using O-Cresolphthalein Complexone (OCPC), Phenol Red, Berthelot and Urea Agar methods. The sensitivity and specificity of the urease methods were obtained and compared.
Results. The sensitivities and specificities obtained were for Phenol Red 97% and 100%, for OCPC 99% and 100%, for Berthelot 97% and 93% and Urea Agar 92% and 96% respectively.
Discussion. The OCPC Method presented the best results of the urease tests evaluated, followed by the Phenol Red method. The OCPC method has the advantage of a greater reagent stability and less possibilities of false positive results due to inadequate washing of the glassware. However the Phenol Red test has a significantly faster reaction time, which is an obvious clinical advantage.


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