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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2000, Number 4

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Rev Biomed 2000; 11 (4)

On-line detection system of Escherichia coli O157:H7: A new support for Public Health

Pacheco-Ferreira A, Martins-Werneck M, Marques-Ribeiro R, Soares d’AP, Cruz ROC, Nascimento-Monteiro TC

Language: English
References: 18
Page: 263-270
PDF size: 69.66 Kb.


ABSTRACT

Introduction. Motivated by countless cases of diarrheic infections occurred in developing countries, an intensity-modulated fibreoptic biosensor was calibrated using Escherichia coli O157:H7.
Material and methods. The device sensitivity has been calibrated for colony forming units (CFU) from 10 to 800 yielding an error less than 11%.
Results. A correlation between optical response and the instantaneous number of bacteria was achieved. The optical output signal was 0.016 (± 0.001) dB per hour per bacteria. In all cases detection started after 270 ± 4 minutes, 5-10 times faster than conventional bacteriological techniques.
Discussion. The results the proposals here presented, can contribute to the real solution of pathogens monitoring and detection, solving many within several problems of Public Health.


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