Rodríguez AJ, Palma S, Maestre JL, Saavedra D, Reyes T, Perovani M

Language: Spanish
References: 19
Page: 257-262
PDF size: 44.61 Kb.

ABSTRACT

Introduction. Mycobacterial diseases are an important health problem worldwide technique based on the Polymerase Chain Reaction (PCR) for the diagnosis of mycobacteria of the complexes Mycobacterium tuberculosis and Mycobacterium avium – intracellulare (MAI), and we compared the obtained results with the results from the conventional diagnosis.
Material and methods. 2 control strains and 15 samples were used: 14 sputum samples and 1 cerebrospinal fluid (CSF) of patients with suspicion of tuberculosis. The samples were processed by the thermic method. Oligonucleotides that amplify the gen of a 65 kDa protein of the complex M. tuberculosis and MAI were used.
Results. Eight samples were positive to the presence of specific mycobacterial DNA and 7 were negative. The results by PCR were corroborated with a mycobacterial culture in 10 cases.
Discussion. This technique reduces the time for the diagnosis of the main mycobacterial diseases.

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