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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2002, Number 4

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Rev Biomed 2002; 13 (4)

Radioimmunoassay data handling

Alvarez-Cervera JH

Language: Spanish
References: 21
Page: 227-287
PDF size: 63.62 Kb.


ABSTRACT

Several procedures for radioimmunoassay (RIA) data handling are described and the basis of Dr. Grafton Chase’s simple approach for the same purpose is also presented. The main features of this method are that it includes an internal quality control system, it does not hide experimental errors since it does not compress the scales of the standard curve, and it enables the identification of suspicious points in the curve. Important factors related to RIA data handling are presented such as: the differences between equilibrium RIA and sequential RIA, sensibility of the assay, antibody specificity, and the verification of quality in the assay using standards or calibrators instead of controls. Examples with typical data supplied by manufacturers of RIA reagents and with data obtained in the laboratory are shown. The use of Dr. Chase’s approach in immunoradiometric assay is also described.


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