2009, Number 2
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Vet Mex 2009; 40 (2)
Comparison of sodium and lithium heparin anticoagulants in plasma biochemistry of Morelet´s crocodiles (Crocodylus moreletii) in Campeche, Mexico
Padilla SE, Weber M, Jacobson E
Language: English/Spanish
References: 19
Page: 203-211
PDF size: 400.36 Kb.
ABSTRACT
Lithium heparin and sodium heparin anticoagulants were compared with the aim to measure concentrations and activity of selected plasma biochemical analytes in wild and captive Morelet’s crocodiles (
Crocodylus moreletii). Blood samples were obtained from the ventral caudal tail vein in 63 crocodiles (32 wild and 30captive) and each sample was placed in both lithium heparin and sodium heparin. Plasma was removed and duplicate samples were assayed for the following: cholesterol, glucose, uric acid, creatinine and alanine aminotransferase (ALT). The only analyte having a significant difference (t = –2.95, df = 56, P ‹ 0.01) when comparing the two anticoagulants was ALT. Still, the difference was not considered to be clinically significant. The results of this study indicates that the type of heparin used as an anticoagulant, does not affect concentratrions and activity of certain plasma biochemicals of Morelet’s crocodiles, and perhaps other species of crocodilians.
REFERENCES
HANLEY CS, HERNANDEZ-DIVERS SJ, BUSH S, LATIMER KS. Comparison of the Effect dipotassium EDTA and Lithium Heparin on Hematologic Values in the Green Iguana (Iguana iguana). J Zoo Wildl Med 2004; 35:328-332.
CAMPBELL TW. Clinical Pathology. In: MADER DR, editor. Reptile Medicine and Surgery. Philadelphia, Pennsylvania: WB Saunders Company 1996: 248-257.
STRIK NI, ALLEMAN AR, HARR KE. Circulating Inflammatory Cells. In: JACOBSON ER, editor. Infectious Diseases and Pathology of Reptiles: Color Atlas and Text. Boca Raton, Florida: CRC Press, 2007: 167-218.
BOLTEN A B, JACOBSON ER, BJORNDAL KA. Effects of anticoagulant and autoanalyzer on blood biochemical values of loggerhead sea turtles (Caretta caretta). Am J Vet Res 1992; 53: 2224–2227.
DEEM SL, DIERENFELD ES, SOUNGUET GP, ALLEMAN AR, CRAY C, POPPENGA RH et al. Blood Values in Free Ranging Nesting Leatherback sea Turtles (Dermochelys coriacea) on the Coast of the Republic of Gabon. J Zoo Wildl Med 2006; 37: 464-471.
CONANP. Programa de Conservación y Manejo Reserva de la Biosfera Los Petenes. México DF: Comisión Nacional de Áreas Naturales Protegidas, 2006.
PLATT SG, THORBJARNARSON JB. Status and Conservation of the American crocodile Crocodylus acutus, in Belize. Biol Cons 2000; 96:13-20.
THORBJARNARSON J, PLATT SG, TUN KHAING SA. Population Survey in the Ayeyarwady Delta, Myanmar. Orix 2000; 34:317-324.
WEBB G, MANOLIS C, SACK GC. Cloacal Sexing of Hatchling Crocodiles. Aust Wildl Res 1984;11:201-202.
PLATT SG, THORBJARNARSON JB. Population Status and Conservation of Morelet’s Crocodile Crocodylus moreletii in Northern Belize. Biol Cons 2000; 96:21-29
SIGLER L, LEÓN-OJEDA F, DOMÍNGUEZ-LASO J, LÓPEZ-OZUNA L, LAVÍN-MURCIO P, Hinojosa-Falcón O. “Muestreos de poblaciones silvestres de Cocodrilos de Morelet Crocodylus moreletii en varios estados de la República Mexicana”. In: SIGLER L, DOMÍNGUEZLASO J, editores. Memorias de la V reunión de trabajo del COMACROM 2002; 2002 noviembre 3-6; Tuxtla (Glutiérrez) Chiapas. Tuxtla (Gutiérrez) Chiapas: ZOOMAT/IHNE, 2002: 120.
JACOBSON ER. Inmobilization, Blood Sampling, Necropsy Techniques and Disease of Crocodilians: a Review. J Zoo Wildl Med 1984; 15:38-45.
LILLYWHITE HB, SMITS AN. Lability of Blood Volume in Snakes and its Relation to Activity and Hypertension. J Exp Biol 1984, 110:267
KÖHLER G. Diseases of Amphibians and Reptiles. Malabar, Florida: Krieger Publishing Company. 2006: 171
JACOBSON ER. Collecting biological samples for clinical evaluation. College of Veterinary Medicine, University of Florida. [Serial online: 2006 Nov] [Cited: 2008 March]. Available from: URL: http//iacuc.ufl .edu/AnimalUseGuides/BiolSampColl.doc
WIENER LAB. Manual del usuario. Rosario, Argentina: Wiener Laboratorios, 2000.
FOWLER J, COHEN L, JARVIS P. Practical Statistics for Field Biology. 2nd ed. London, UK: John Wiley and Sons, 2006.
KELLER JM, MCCLELLAN-GREEN PD, LEE AM, ARENDT MD, MAIER PP, SEGARS AL et al. Mitogeninduced lymphocyte proliferation in loggerhead sea turtles: comparison of methods and effects of gender, plasma testosterone concentrations and body condition on immunity. Vet Immunol Immunopathol 2005; 103:269-281.
FRYE FL. Hematology as Applied to Clinical Reptile Medicine. In: FRYE FL, editor. Reptile Care: An Atlas of Diseases and Treatments. Vol 1. New Jersey: TFH Publications INC, 1991: 209-262.