medigraphic.com

2009, Number 2

<< Back Next >>

Vet Mex 2009; 40 (2)

Immunohistochemical diagnosis of immunemediated dermatoses in domestic dogs

Bedolla AMA, Salas GG, Constantino CF

Language: English/Spanish
References: 21
Page: 181-189
PDF size: 536.54 Kb.


ABSTRACT

A retrospective study of 28 dogs skin samples was carried out between 2001 and 2004. The fixed paraffin-embedded tissues showed microscopic lesions and suggestive diagnoses of pemphigus or lupus erythematosus (LE). The samples were re-evaluated by immunohistochemistry to identify intratissular deposits of canine IgG. Only 57.14% (16 cases) were positive. The location of IgG in the interstice space of the epidermis or in the basal membrane was helpful to differentiate between pemphigus and lupus erythematosus and among the different forms of pemphigus. From the positive cases 50% (eight cases) were consistent with pemphigus foliaceus, 31.25% (five cases) with pemphigus vulgaris, 12.5% (two cases) with pemphigus erythematosus, and 6.25% (one case) with lupus erythematosus. Immunohistochemistry is a valuable diagnostic tool for the definite diagnosis of immune mediated diseases such as pemphigus and lupus erythematosus. The aim of the present study was to demonstrate the usefulness of a never used method in Mexico for the specific diagnosis of pemphigus and lupus erythematosus.


REFERENCES

  1. SCOTT DW, MILLER WH, GRIFFIN CE. Immunologic skin disease In: SCOTT DW, MILLER WH, GRIFFIN CE, editors. Muller and Kirk’s small animal dermatology. 5th ed. Philadelphia: WB Saunders Company, 1995: 556-588.

  2. SCOTT DW, WALTON DK, SLATER MR, SMITH CA, LEWIS RM. Immune-mediated dermatosis in domestic animals: Ten years after-Part I. Comp Cont Educ 1987; 9: 424-435.

  3. SCOTT DW, WALTON DK, SLATER MR, SMITH CA, LEWIS RM. Immune-mediated dermatosis in domestic animals: Ten years after-Part II. Comp Cont Educ 1987; 9: 539-551.

  4. TIZARD IR. Organ specific autoimmmune diseases In: TIZARD IR, editor. Veterinary immunology. An introduction. 6th ed. Philadelphia: WB Saunders Company, 2000: 375-377.

  5. LEE GT. Superficial pustular diseases of the epidermis en Veterinary dermatopathology In: LEE GT, editor. Veterinary dermatopathology. St. Louis: Mosby, 1992: 16-20.

  6. HURVITZ AI, FELDMAN E. A disease in dogs resembling human pemphigus vulgaris: case reports. J Am Vet Mec Assoc 1975; 166: 585-590.

  7. YAGER JA, SCOTT DW. The skin appendages In: JUBB KVF, KENNEDY PC, PALMER,N, editors. Pathology of domestic animals. Vol. I. 4th ed. New York: Academic Press, 1993: 617-623.

  8. LEE GT. Bullous and vesicular diseases of the epidermis and dermal-epidermal junction In: LEE GT, editor. Veterinary dermatopathology. St. Louis: Mosby, 1992: 22-33.

  9. TIZARD IR. The systemic immunological diseases In: TIZARD IR, editor. Veterinary immunology. An introduction. 6th ed. Philadelphia: WB Saunders Company, 2000: 386-390.

  10. SCOTT DW, WALTON DK, MANNING TO, SMITH CA, LEWIS RM. Canine lupus erythematosus. I. Systemic lupus erythematosus. J Am Anim Hosp Assoc 1983; 19: 461-479.

  11. TIZARD IR. Diagnostic Applications of immunological test In: TIZARD IR, editor. Veterinary immunology. An introduction. 6th ed. Philadelphia: WB Saunders Company, 2000: 386-390.

  12. HPROPHET EB, MILLS B, ARRINGTON JB, SOBIN L. Métodos histotecnológicos. Washington DC: Ed. Registro de patología de los Estados Unidos de América, 1993.

  13. HAINES DM, COOKE EM, CLARK EG. Avidin-Biotin- Peroxidase complex immunohistochemistry to detect immunoglobulin in formalin fixed skin biopsies in canine autoimmune skin disease. Can J Vet Res 1987; 51: 104-109.

  14. BRADLEY GA, CALDERWOOD MB. Immunoperoxidase staining for the detection of autoantibodies in canine autoimmune skin disease; comparison to immunofluorescence results. Vet Immunol Immunopathol 1990; 26: 105-113.

  15. PEREZ J, ARCE C, MORENO A, MOZOS E, RODRIGUEZ F, LLANES D. Comparison of three monoclonal and three policlonal antibodies in the immunohistochemical diagnosis of canine autoimmune skin diseases. Vet Dermatol 2002; 13: 231-236.

  16. TIZARD IR. Antibodies: soluble forms of BCR In: TIZARD IR, editor. Veterinary immunology. An introduction. 6th ed. Philadelphia: WB Saunders Company, 2000: 139-148.

  17. SCOTT DW, MANNING TO, SMITH CA, LEWIS RM. Observations on the immunopathology and therapy of canine pemphigus and pemphigoid. J Am Vet Med Assoc 1982; 180: 48-52.

  18. HAROLD E, LANE D. Cell staining. In: HAROLD E, LANE D, editors. Antibodies a Laboratory Manual. New York: Cold Spring Harbor Laboratory, 1988: 384-389.

  19. RAMOS-VARA JA, KIUPEL M, BASZLER T, BLIVEN L, BRODERSEN B, CHELACK B et al. Suggested guidelines for immunohistochemical techniques in veterinary diagnostic laboratories. J Vet Diagn Invest. 2008; 20: 393-413.

  20. DAY MJ, HANLON L, POWELL LM. Immune-mediated skin disease in the dog and cat. J Comp Path 1993; 109: 395-407.

  21. ROSENKRANTZ WS. Pemphigus: current therapy. Vet Dermatol 2004; 15: 90-98.




2020     |     www.medigraphic.com