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2008, Number 4

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Vet Mex 2008; 39 (4)

The Fasciola hepatica excretory-secretory products reduce the production of cells producing antibodies against thymus-dependent antigens in mice

Bautista GCR, Lebrija RA

Language: English/Spanish
References: 21
Page: 429-433
PDF size: 145.52 Kb.


ABSTRACT

The aim of the present study was to evaluate the effect of adult Fasciola hepatica excretion-secretion products administered intraperitoneally (ip) to NIH mice, on the number of cells producing antibodies against sheep erythrocytes (thymus dependent antigen). Thirteen mice were distributed at random into two groups which were treated ip, daily during seven days, with either phosphate buffered saline (Group I: PBS, n = 6) or excretion-secretion products of adult Fasciola hepatica (Group II: ESP_Fh, n = 7). At day eight, each mouse in both groups was immunized ip with sheep erythrocytes and four days later, the number of plaqueforming cells (PFC) in a suspension of spleen cells per each mouse was recorded. The mean number (± SEM) of PFC obtained in the group PBS was signifi cantly higher (72 ± 10; P ‹ 0.01) than the value observed in the group ESP_Fh (27 ± 9). The results indicate that ESP_Fh induce immunosupression to thymus dependent antigens in mice.


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