Durán-Monterrosas L, Flores-Reyes A, Vega-Hernández E, Echeverría-Sánchez M, Villanueva-Díaz CA

Language: Spanish
References: 29
Page: 168-175
PDF size: 305.79 Kb.

ABSTRACT

Objective: The aim of present study was to analyze the effects of human serum albumin (HSA) added to the sperm preparation medium on sperm viability of semen samples prepared for intrauterine insemination.
Material and methods: Fifteen semen samples from healthy donors with normal semen (WHO criteria) were divided in two aliquots and processed by the swim-up method using either Human Tubal Fluid (HTF) or HTF added with 10% HSA for separation of the motile fraction of sperm. At the end of sperm preparation and 24 hours later; sperm motility parameters were compared using the Hamilton-Thorn IVOS 2000 semen analyzer. Progressive motility was used as a measure of viability in the samples.
Results: VSL decreased from 17.14 ± 5.77 um/seg (mean ± SD) to 12.21 ± 3.86 um/seg and 12.14 ± 3.13 um/seg in the samples processed with and without 10% HSA, respectively. At 24 hours progressive motility decreased 11.41% in aliquots processed with HTF-HSA and 21.75% in those prepared with HTF (z = 1.88; p =0.05). HOST test results of HTF and HTFHSA treated samples were 88.85% ± 6.90% and 91.92% ± 4.55% respectively (p = 0.02). Acrosomal integrity was also higher in samples processed with HSA (86.14% ± 3.08% vs. 81.57% ± 2.53%; p ‹ 0.01).
Conclusions: These data suggest that HSA could help preserve some of the molecules related to sperm motility and enhance sperm survival.

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