Gómez-Rivera N, Molina MAF, García ZMG, Castillo AJD, Castillo RJ, García HRJ, Fonseca CI, Valenzuela AO

Language: Spanish
References: 18
Page: 109-112
PDF size: 57.34 Kb.

ABSTRACT

Objective. To compare the effectiveness of stain technique using the dyes hematoxylin and eosine (H/E) for the identification of Entamoeba histolytica/E. dispar, with the well known, direct observation technique of fresh stool sample (FSS) taken in children with acute diarrhea (AD).
Material and methods. The samples of stool of 137 children with diarrhea were prepared in a smear with a drops of lugol solution. The microscopy observation of the feces was done in fresh samples of stools, before 30 minutes. Simultaneously amoeba was looked in fresh stools by the stain technique with H/E. The positive cases were related according to the clinical presumption of amebiasis.
Results. With the stain technique H/E were found positives 35 smears obtained in 137 children. Using the other technique only 5 cases were positives (p ‹ 0.001) in the same children. There was a clinical presumption of amebiasis in 52 children, in 29 of them it was observed the presence of erithrophagocytosis in the smear study.
Conclusions. The stain contrast technique of feces, using H/E, show high effectiveness in order to observed the Entamoeba histolytica erithrophagocytosis.

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