2001, Number 1
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Rev Biomed 2001; 12 (1)
Molecular and antigenic characterisation of rotavirus associated with an outbreak of acute infection diarrhea at a nursery in Merida, Yucatan, Mexico.
Puerto-Solís M, Polanco-Marín G, Manzano-Cabrera L, Cámara-Mejía J, González-Losa MR, Taniguchi K
Language: English
References: 30
Page: 5-10
PDF size: 35.16 Kb.
ABSTRACT
Introducción. Rotavirus (RV), is a major cause of acute infectious diarrhea (AID) in children. The profile genomic and antigenic studies of RV are important to determine the molecular changes that the strains have been presenting presented through the years. The purpose of this study was to carry out the electrophoretic and antigenic characterization of RV strains circulating during an outbreak of AID at a nursery in Merida, Yucatan, Mexico in January, 1996.
Material and methods. Faecal samples were obtained from 29 children and 7 adults and were analysed using a polyacrylamide gel electrophoresis (Page) techique and Elisa with monoclonal antibodies for subgroup, G and P serotypes.
Results. A total of 61.1% of the samples from children and adults were found to be positive for Group A RV. Subgroups I and II were found in a similar proportion. G1 serotypes were detected in 50%, G2 in 50% and P1A serotypes in 100% of the samples. 50% of the viral RNA electrophoretical patterns, were short patterns and 50% were long patterns. The serotype G2, subgroup I, short patterns and one child who required hospitalization due to acute dehydratation.
Discusion. Since the nursery is a closed place the presence of two different strains, indicates that two different strains were circulating at the same time. Short pattern, G2 serotypes and subgrup I were found in 50% of the samples, and this had not been observed in the Yucatán since 1989. Our results suggest that short pattern serotype G2, subgroup I are associated with more serious clinical manifestations.
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