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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2003, Number 1

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Rev Biomed 2003; 14 (1)

Evaluation of a competitive enzime linked immunosorbant assay for the serological diagnosis of bovine brucellosis

Dájer-Abimerhi A, Gutiérrez-Ruiz EJ, Zapata-Villalobos DM, Sierra-Lira EM, Cámara-Gamboa EI

Language: Spanish
References: 19
Page: 23-28
PDF size: 37.16 Kb.


ABSTRACT

Introduction. Brucellosis is a worldwide zoonotic disease, that causes losses to cattle production, so its necessary to have simple, sensitive and specific diagnostic tests to establish effective prevention and control measures.
Material and Methods. 724 bovine sera were tested with an experimental competitive enzime linked immunoabsorbant assay (C-ELISA); the cut-off point was determined using a ROC analysis and a scattergram. Agreement between the C-ELISA and the complement fixation test (CFT) was determined with the Kappa test. Sensitivity and specificity were also determined.
Results. The optimum cut off-point was 47% inhibition.Agreement between C-ELISA and CFT was 0.8 for all sera, 0.84 for sera from non vaccinated animals and 0.72 for sera from vaccinated animals. Sensitivities were 92.27% (confidence intervals 95% (CI) 88.51-96.03) for all sera; 93.86% (CI 95% 84.45-98.27) for sera from non vaccinated animals and 90% (CI 95% 83.43-96.57) for sera from vaccinated animals. Specificities were 91.89% (CI 95% 89.56-94.21) for all sera; 94.26% (CI 95% 92.05-96.49) for sera from non vaccinated animals and 83.04% (CI 95% 76.08-89.99) for sera from vaccinated animals.
Discussion. The optimum cut-off determined was different from that recommended by the kit producer. Agreement between C-ELISA and CFT was high but the sensitivities and specificities with the 47% inhibition cut-off point do not make the C-ELISA a better test than CFT. However, moving the cut-off point can improve sensitivity or specificity depending on the objectives of the control/erradication compaing for bovine brucellosis.


REFERENCES

  1. Alvarez E. Situación de la brucelosis en América: Panorama general. En: SAGAR-UNAM editores. Memorias del III Foro Nacional de Brucelosis; 1998. pp 23-31.

  2. Corbel MG. Brucelosis. En: Laing JA, Brinkley WR, Wagner WC, editores. Fertilidad e Infertilidad en la práctica veterinararia. 4a ed. México: Interamericana-McGraw-Hill; 1996. pp. 201-36.

  3. Bernués A, Manrique E, Maza M. Economic evaluation of bovine brucellosis and tuberculosis erradication programmes in a mountain area of Spain. Prev Vet Med 1997; 30:137-49.

  4. López MA, López SR, Ocampo DA, González DI. Brucelosis avances y perspectivas. México: INDRESubsecretaría de Coordinación y Desarrollo- SSA; 1991.

  5. Diario Oficial . Normas de la Campaña Nacional contra la Tuberculosis y Brucelosis en México. México; 1996, pp. 45-66.

  6. Central Veterinary Laboratory (CVL). Standard Laboratory Techniques for Brucellosis Diagnosis. Inglaterra (Weybridge): Ministry of Agriculture, Fisheries and Food; 1991.

  7. Mejía TC, Luna ME. Descripción y generalidades de la brucelosis. En: Secretaria de Agricultura y Ganaderia Rural-Escuela de Médicos Veterinarios Zootecnistas editores. Manual de actualización técnica para la aprobación del médico veterinario como unidades de verificación en tuberculosis bovina y brucelosis. México; 1996. p. 43-46.

  8. Dájer AA, Gutiérrez RE, Zapata VD, Villegas PS. Uso de las pruebas de ensayo inmunoabsorbente ligado a enzimas y aglutinación con Rivanol para el diagnóstico de Brucelosis Bovina en Yucatán.Vet Méx 1998; 29: 167-71.

  9. Dájer-Abimerhi A, Gutiérrez-Ruiz E, Zapata-Villalobos D, Honhold N, Villegas-Pérez S. Comparación de cinco pruebas serológicas para la detección de anticuerpos contra Brucella abortus y reporte preliminar del porcentaje de reactores positivos en hatos bovinos en Yucatán, México. Rev Biomed 1995; 6:84-90.

  10. Wright PF, Nielsen KH, Kelly WA. Primary binding techniques for the serodiagnosis of bovine brucellosis: Enzyme immunoassay. En Nielsen KH, Duncan JR, Editores. Animal brucellosis. Boca Ratón: CRC Press; 1990. p. 199-235.

  11. Nielsen K, Gall D, Kelly W, García M. Enzyme immunoassay, application to diagnosis of bovine brucellosis. Canadá; Animal Diseases Research Institute, Agriculture and Agri-Food Canada: 1992. pp 35-43.

  12. Nielsen KH, Kelly L, Gall D, Nicoletti P, Kelly W. Improved competitive immunoassay for the diagnosis of bovine brucellosis, Vet Immunol Immunopath 1995; 45:285-91.

  13. Center Disease Control. Epi Info Versión 6.1. Organización Mundial de la Salud. E.U.

  14. Microsoft Corporation. Excel Versión 6.0. E.U.

  15. Animal Diseases Research. Med Calc Versión 3.0. Belgium.

  16. Martín W, Meek A, Willenberg P. Veterinary epidemiology: Principles and methods. Iowa: Iowa State Press; 1987. p. 343.

  17. Agger JF, Noordhuizen J, Willeberg P, Van Voorthuysen PF. Episcope Versión 1.0. Agricultural University. Netherlands.

  18. Blood DC, Henderson JA, Radostitis OM. Medicina Veterinaria. 6a ed. México: Interamericana; 1986. pp 662-673.

  19. Food and Agricultural Organization (FAO)/World Health Organization (WHO)/Organization International of Epizoties (OIE). Report 1986: Joint FAO/WHO Expert Committee on Brucellosis, EUA : WHO Technical Report Series; 1986.




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