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Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2003, Number 1

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Rev Biomed 2003; 14 (1)

Detection of HBsAg in eluates of blood spotted on filter paper using the UMELISA HBsAg PLUS

Delahanty-Fernández A, Valdivia-Alvarez I, Ventura-Paz J, Trujillo-Brito J, Ortega-León D, Palenzuela-Díaz A

Language: Spanish
References: 13
Page: 17-21
PDF size: 57.20 Kb.


ABSTRACT

Introduction. The enzyme immunoassays have been employed in the detection of HBsAg using samples of dry blood on filter paper due to the advantages of this type of samples, since it diminishes the use of materials of laboratories, this type of samples can be used in those countries where the resources are limited to carry out epidemic studies.
Material and methods. In this work we established the good conditions for the elution of the samples of dry blood on filter paper with the purpose of carrying out the detection of HBsAg using the UMELISA HBsAg PLUS. 1053 samples of serum, and dry blood were evaluated on filter paper, coming from blood donors of Havana City, Cuba.
Results. In the samples of serum, 9 individuals were found reagent and 8 out of the 9 were confirmed ace positive by the rehearsal HBsAg confirmatory test for 0,75 % and in those of dry blood on filter paper, 6 out of those confirmed were reagent, what represents 0,57%.
Conclusions. The results for both types of samples were compared and the index Kappa was of 0,85%, for a good agreement.


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