medigraphic.com
Centro de Investigaciones Regionales Dr. Hideyo Noguchi, Universidad Autónoma de Yucatán

2008, Number 3

<< Back Next >>

Rev Biomed 2008; 19 (3)

Antimicrobial susceptibility and virulence factors of Vibrio cholerae no-O1 isolates isolated from patients with acute diarrhea

Cabrera-Rodríguez LE, Bravo-Fariñas L, Ramírez-Álvarez MM, Llop-Hernández A, Fernández-Abreu A, Morier L, Borrego-Hernández G

Language: Spanish
References: 37
Page: 138-144
PDF size: 204.00 Kb.


ABSTRACT

Introduction. The intestinal infections caused for Vibrio cholerae no-O1 are increasing in the world.
Objective. To know the antimicrobial susceptibility to the drug of choice and alternative ones and to determine the presence of some virulence factors as: extracellular enzymes (Dnase, gelatinase, lecitinase, elastase and hemolysin), HEp-2 cells adherence and presence of fimbrias.
Materials and Methods. A total of 65 isolates of Vibrio cholerae no-O1 were obtained from patients with acute diarrhea in 7 provincial laboratories of microbiology in Cuba from January to December 2006.
Results. The figures for resistance of sulphonamide and ampicillin were 32.3% and 30.7%, respectively. Sensitivity levels over 85% were recorded for the antibiotics fluoroquinolones, tetracycline, chloramphenicol and trimetoprimsulphamethoxazole. Hemolysin and gelatinase enzymes were found in 100% of the isolates.
Conclusions. The studied isolates show low resistance towards the tested antimicrobials except for sulfonamide and ampicilline. At least two of the investigated virulence factors were found in all isolates.


REFERENCES

  1. Garrity GM, Bell JA, Lilburm TG. Taxonomic outline of the prokaryotes. En: Bergey ′s Manual of Systematic Bacteriology. 2 nd ed. Release 4.00; Oct 2004. [Citado el 10 de febrero del 2005]. Disponible en URL: http://dx.doi.org/10.1007/bergeysoutline

  2. Lesmana M, Subekti DS, Tjaniadi P, Simanjuntak CH, Punjabi NH, Campbell JR, et al. Spectrum of Vibrio species associated with acute diarrhea in North Jakarta, Indonesia. Diagn Microbiol Infect Dis 2002; 43:91-7.

  3. Chatterjee SN, Chaudhuri K. Lipopolysaccharides of Vibrio cholerae. I. Physical and chemical characterization. Biochem Biophys Acta 2003; 1639:65-79.

  4. Nair GB, Oku Y, Takeda Y, Ghosh A, Ghosh RK, Chattopadhyay S, et al. Toxin profiles of Vibrio cholerae non-O1 from environmental sources in Calcutta, India. Appl Environ Microbiol 1988; 54:3180-2.

  5. Farfan M, Miñana D, Fusté MC, Lorén JG. Genetic relationships between clinical and environmental Vibrio cholerae isolates based on multilocus enzyme electrophoresis. Microbiology 2000; 146:2613-26.

  6. Shinoda S. Protein toxins produced by pathogenic vibrios. J Nat Toxins 1999; 8:259-69.

  7. Bhattacharya MK, Dutta D, Bhattacharya SK, Deb A, Mukhopadhyay AK, Nair GB, et al. Association of a disease approximating cholera caused by Vibrio cholerae of serogroups other than O1 and O 139. Epidemiol Infect 1998; 120:1-5.

  8. Boyd EF, Waldor MK. Alternative mechanism of cholera toxin acquisition by Vibrio cholerae: generalized transduction of CTXФ by bacteriophage CP-T1. Infect Immun 1999; 67:5898-905.

  9. Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standarized single disk method. Am J Clin Pathol 1966; 45:493-6.

  10. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing: eleventh informational supplement. Wayne; 2005. CLSI Document M100-S15.

  11. Mc Faddin JF. Pruebas bioquímicas para la identificación de bacterias de importancia clínica. Baltimore: William and Wilkins; 2002.

  12. Scharmann W. Elastase in Pseudomonas and Aeromonas Zentralbl Bakteriol 1972; 220:435-42.

  13. Blazevic DJ, Ederer GM. Principles of biochemical test in diagnostic microbiology. New York, J. Wiley. 1975.

  14. Boiko AV. Pathogenicity factors of various Vibrios and Aeromonas. Zh Mikrobiol Epidemiol Immunobiol 2000; 79:104-8.

  15. Robinson J, Beaman J, Wagener L, Burke V. Comparison of direct plating with the use of enrichment culture for isolation of Aeromonas spp from faeces. J Med Microbiol 1986; 22:315-7.

  16. Carrello A, Solburn K, Budden J, Chong B. Adhesion of clinical and environment Aeromonas isolates to HEp-2 cells. J Med Microbiol 1988; 26:19-27.

  17. Nishikawa Y, Kimiura T, Kishi T. Mannose-resistant adhesion of motile Aeromonas to INT 407 cells and the differences among isolates from humans food and water. Epidemiol Infect 1991; 107:171-9.

  18. Iwanaga M, Toma C, Miyazato T, Insisiengmay S, Nakasorte N, Ehara M. Antibiotic resistance conferred by a class I integron and SXT constin in Vibrio cholerae O1 strains isolated in Laos. Antimicrob Agents Chemother 2004; 48:2364-9.

  19. Mohanthy S, Kapil A, Das BK. Seasonality and antimicrobial resistance pattern of Vibrio cholerae in a tertiary care hospital of North India. Trop Doct 2004; 43:249-51.

  20. Morris JG, Tenney JH, Drusmo GL. In vitro susceptibility of pathogenic Vibrio species to norfloxacin and six other antimicrobial agents. Antimicrob Agent Chemother 1985; 28:442-5.

  21. Tjaniadi P, Lesmana M, Subekti D, Machpud N, Komalarini S, Santoso W. Antimicrobial resistance of bacterial pathogens associated with diarrheal patients in Indonesia. Am J Trop Med Hyg 2003; 68:666-70.

  22. Bravo L, Cabrera L, Ramírez M, Castañeda N, Fernández E, Garrigó E. Sensibilidad antimicrobiana de cepas de Vibrio cholerae no-O1 aisladas de pacientes en Cuba. Rev Esp Quimioterap 2004; 17:200-1.

  23. Bravo L, Ramírez M, Maestre JL, Llop A, Cabrera R, García B. Vibrio cholerae no-O1 toxigénico. Rev Cubana Med Trop 2000; 52:106-9.

  24. Ibarra JO, Flores LE, García RH, Alvarado T. Estudio preliminar de la resistencia de Vibrio cholerae O1, brote epidémico 1998, a antimicrobianos. Rev Peru Biol 2000; 7:3-6.

  25. Neelam K, Manjula M, Vikas G, Varsha G. Outbreak of cholera in & around Chandigarh during two successive years (2002,2003). Indian J Med Res 2005; 122:404-407.

  26. Organización Panamericana de la Salud. Resistencia antimicrobiana en las Américas: Magnitud del problema y su contención. Washington DC: OPS; 2003.

  27. World Health Organization. Management of the patient with cholera. [Citado el 20 de julio del 2005]. Disponible en URL: http://www.who.int/csr/resources/publications/cholera/whocddser9115rev1.pdf.

  28. Bidinost C, Saka HA, Aliendro O, Sola C, Panceta-Dutttari G, Carranza P, et al. Virulence factors of non-O1 non-O139 Vibrio cholerae isolated in Córdoba, Argentina. Rev Argentina Microbiol 2004; 36:158-63.

  29. Sack D, Sack R, Nair G, Siddique A. Cholera. Lancet 2004; 363:223-33.

  30. Singh DV, Matte MH, Matte GR, Jiang S, Sabena F, Shukla BN. Molecular analysis of Vibrio cholerae O1, O139, non-O1, and non-O139 strains: Clonal relationships between clinical and environmental isolates. Appl Environ Microbiol 2001; 67:910-21.

  31. Israll AM, Balotescu C, Lazar V, Cernat R, Dinu C. Factors influencing the capacity of cellular substrate adherencia of Vibrio cholerae O1 and non O1 strains. Bacteriol Virusol Parazitol Epidemiol 2002; 47:119-24.

  32. Dalsgaard A, Albert MJ, Tavior DN, Shimada T, Meza R, Serichantelergs O, et al. Vibrio cholerae non-O1 serogrups obtained from an outbreak of diarrhea in Lima, Peru. J Clin Microbiol 1995; 33:2715-22.

  33. Israll A, Balotescu C, Camian M, Dinu C, Bucurenci N. Comparative study of different methods for detection of toxic and other enzymatic factors in Vibrio cholerae strains. Roum Arch Microbiol Immunol 2004; 63:63-77.

  34. Chiavelli DA, Marsh JW, Taylor RK. The Mannose–sensitive hemagglutinin of Vibrio cholerae promotes adherence to zooplankton. Appl Environm Microbiol 2001; 67:3220-5.

  35. Mathur J, Waldor MK. The Vibrio cholerae ToxRregulated porin OmpU confers resistance to antimicrobial peptides. Infect Immun 2004; 72:3577-83.

  36. Telesmanich NR, Bezuglova EV, Mishankin MB, Vinokur NI. Role of lectin (hlyA) in the hemotytic and hemaglutinating activity Vibrio cholerae. Zh Microbiol Epidemiol Immunobiol 2004; 5:12-6.

  37. Gibotti A, Saridakis HO, Pelayo JS, Tagliari KC, Paseto Falcão D. Prevalence and virulence properties of Vibrio cholerae non-O1, Aeromonas spp. and Plesiomonas shigelloides isolated from Cambé Stream (State of Paraná, Brazil). J Appl Microbiol 2000; 89:70-5.




2020     |     www.medigraphic.com