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ISSN 0187-5337 (Print)
Instituto Nacional de Perinatología

2002, Number 4

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Perinatol Reprod Hum 2002; 16 (4)

El líquido peritoneal de mujeres con endometriosis promueve la adhesión celular. Modelo in vitro

Hernández-Guerrero C, Vadillo OF, Jiménez-Zamudio L, Tlapanco-Barba R, González-Jiménez M, Ramos-Pérez C, Julio de la Jara-Díaz

Language: Spanish
References: 27
Page: 163-171
PDF size: 266.53 Kb.


ABSTRACT

Objective; To evaluate adhesion cellular capacity of the peritoneal fluid from women with endometriosis.
Material and methods: Seventy percent ethanol and ice were used to denudated amniotic membrane epithelia cells, from human chorioamnios membranes obtained by delivery at term. Wish (amniotic derivate epithelial cell line) cells were used to assignment cellular adhesion; cells were target with 100 mCi/mmol thymidine (3H) pulse. Around 3 x105 target cells were added to a 10 mm amnion explants diameter by 60 minutes, at optimal culture cell conditions, measuring cell adhesion every 10 minutes.
Results: Cell adhesion in an in vitro model was determined by beta-radioactive measures at the explants. Basal cellular adhesion start at the first ten minutes until sixty minutes, that shows around 5% media cellular adhesion at every kinetic step. Cells incubated whit peritoneal fluid (PF) from endometriosis womwn, shows cellular adhesion increment at all kinetic steps (ANOVA F =30.0; IC 95%, p ‹ 0.05) versus value showed by control womwn LP, or fetal bovine sera used at the same protein concentration.
Conclusions: Work present here describes an in vitro model to evaluate cell adhesion; and shows different capacity between LP from endometriosis sterile and fertile women to promotes cell adhesion.


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