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Coordinación General de Investigación de la Facultad de Salud Pública y Nutrición y la Dirección General de Sistemas e Informática de la Universidad Autónoma de Nuevo León

2008, Number 4

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Rev Salud Publica Nutr 2008; 9 (4)

Potencial probiótico de cepas de lactobacillus aisladas de quesos ovinos patagónicos

Vallejo M, Marguet ER, Etchechoury VE

Language: Spanish
References: 28
Page:
PDF size: 104.37 Kb.


ABSTRACT

In the present work the probiotic potencial of 20 strains of Lactobacillus spp. isolated from ovine milk cheese from Valle Inferior del Río Chubut, (Patagonia-Argentina) was evaluated. The strains were examined in relation to acidic and bile salts tolerance, antimicrobial activities and b-galactosidase (b-Gal) activity, and were comparated with commercial strains. Microbial adhesion to hydrocarbons and autoagregation ability on selected strains were also studied. Survival following 4 h of incubation at pH 1.0 was observed for 10 strains, 7 strains survived to pH 2.0, 2 strains to pH 2.5, whereas 1 strain could not survive any of the treatments. In all studied strains the duplication rate in the presence of 0.3 % bile salts was not affected. Twelve strains displayed antimicrobial activities against all Gram-positive and Gram-negative bacteria studied. b-Gal activity was not detected in 6 strains, whereas the remanining strains displayed values comparable with those obtained in commercial ones. From the results obtained in previously described tests, 5 strains were selected to evaluate the adherence to hydrocarbons. Strains Lactobacillus LbTw3, LbTw5 and LbTw7 showed values higher than 78 % and were subjected to autoaggregation test. The results of the test suggest a strong relationship between autoaggregation and cell surface hydrophobicity. On the basis of the results obtained, we concluded that strains LbTw3, LbTw5 and LbTw7 exhibit characteristics to be regard as potencially probiotic strains. Since microorganisms do not need to fulfil all selected criteria, the remainig strains could be sujected to further tests in order to study additional properties.


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