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Coordinación General de Investigación de la Facultad de Salud Pública y Nutrición y la Dirección General de Sistemas e Informática de la Universidad Autónoma de Nuevo León

2008, Number 3

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Rev Salud Publica Nutr 2008; 9 (3)

Detección, caracterización serológica y antibiogramas de Escherichia coli aisladas de carne de ternera (babilla) entera y picada

Maria KC, Maia FR, Trindade OLA, Valente AM, Carvalho JCAP, Conte-Junior CA

Language: Spanish
References: 43
Page:
PDF size: 122.29 Kb.


ABSTRACT

The outbreaks of foodborne diseases constitute a target of concern to the food industries and to the public health agencies. Pathogenic variants of Escherichia coli are usually present in the intestinal tract of animals, making possible the contamination of the carcass and meat cuts during slaughtering or by inadequate processing. The importance of meat as human food associated to the needs of having a safety food which is not capable to cause illnesses, had induced to the development of this research with the objective to detect, to characterize the serogrupos and to make antibiograms of strains of E. coli. Thirty samples of beef (heart of rump) sold in supermarkets and butchers of Rio de Janeiro (Brazil) were analysed. Of these samples, 15 were composed by whole beef and 15 were grounded in each own establishment. Different methodologies for the biochemical and serologically confirmation were used. After identification, the strains were also tested for antibiotic susceptibility. In relation to the samples analysed, 100% of them were contaminated with total coliforms and five of them (16.7%) did not detect fecal coliforms. The count of total coliforms and E. coli fluctuated from 4.0x103 to 1.1x106 and 0 to 2.4x103 in whole meat, respectively, whereas in ground beef, it was from 4.4x103 to 2.5x107 and 0 to 3.0x105, respectively. In relation to the methodology used, the second method was more efficient than the third method, because it presented a greater number of confirmed colonies. Using the second method, we isolated 52 strains of enteropathogenic E. coli (EPEC), eight strains of enteroinvasive E. coli (EIEC) and one strain of enterohemorrhagic E. coli (EHEC). The strains of E. coli isolated and characterized as pathogenic displayed great spectrum of antibiotic resistance, mainly to ampiciline (11.32%), cefalotin (11.11%) and amicacine (10.06%). The presence of the serogroups of E. coli in the examined samples stands out the need of programs of Good Manufacturing Practice (GMP), Sanitation Standard Operation Procedures (SSOP), and Hazard Analysis and Critical Control Points (HACCP); to preserve the consumer’s health.


REFERENCES

  1. Felício, P.E. 1998. Desdobramento da qualidade de carne bovina. Hig. Aliment. Vol. 12 No. 54: 16-22.

  2. Gill C.O., J. Bryant and C. Landers 2003. Identification of critical control points for control of microbiological contamination in processes leading to the production of ground beef at a packing plant. Food Microbiol. 20 (6): 641-650.

  3. Barkocy-Gallagher G.A., K.K. Edwards, X. Nou, J.M. Bosilevac, T.M. Arthur, S.D. Shackelford, and M. Koohmaraie 2005. Methods for recovering Escherichia coli O157:H7 from cattle fecal, hide, and carcass samples: sensitivity and improvements. J. Food Prot. 68 (11): 2264–2268.

  4. Marzocca M.A., P.L. Marucci, M.G. Sica y E.E. Álvarez 2006. Detección de Escherichia coli O157:H7 encarne picada fresca y hamburguesas congeladas. Rev. Argent. Microbiol. Vol. 38: 38-40.

  5. Brasil 2002. Manual das doenças transmitidas por alimentos. Escherichia coli O157:H7 – enterohemorrágica. Centro Brasilerio de Vigilância Sanitária – Divisão de Doenças e Transmissão Hídrica e Alimentar. São Paulo. 126 pp.

  6. Estrada-García T., J.F. Cerna, L. Paheco-Gil, R.F. Velázquez, T.J. Ochoa, J. Torres and H.L. DuPont 2005. Drug-resistant diarrheogenic Escherichia coli, México. Emerging Infect. Dis. 11(8): 1306-1308.

  7. Barlow R.S., K.S. Gobius and P.M. Desmarchelier 2006. Shiga toxin-producing Escherichia coli in ground beef and lamb cuts: results of a one-year study. Int. J. Food Microbiol. 111(1): 1-5.

  8. Hobbs, B.C. e D. Roberts 1999. Toxinfecções e controle higiênico-sanitário de alimentos. Editorial Varella 425pp.

  9. Franco, B.D.G.M e M. Landgraf 1996. Microrganismos patogênicos de importância em alimentos. En Microbiologia de Alimentos. [B.D.G.M Franco e M. Landgraf] Ed. Atheneu. Cap. 4: p.33-82.

  10. Buchanan, R.L. and M.P. Doyle 1997. Foodborne disease significance of Escherichia coli O157:H7 and other enterohemorrhagic Escherichia coli. Food Technol. 51 (10): 69-76.

  11. Merck 2000. Diagnóstico rápido de E. coli com os meios de cultura Fluorocult. Editorial Merk 382pp.

  12. Mehlman, I.J. and J. Lovett 1984. Enteropathogenic Escherichia coli. In Bacteriological analytical manual. Editorial Association of Official Analytical Chemists p.6.01.

  13. Toledo M.R.F., C.F. Fontes e L.R. Trabulsi 1982. Mili- Um meio para realização dos testes de motilidade, indol e lisina descarboxilase. Rev. Microbiol. Vol.13 No. 3: 230-235.

  14. Toledo M.R.F., C.F. Fontes e L.R. Trabulsi 1982. EPM- Modificação do meio de Rugai e Araújo para realização simultânea dos testes de produção da gás a partir da glicose, H2S, urease e triptofano desaminase. Rev. Microbiol. Vol.13 No. 4: 309-315.

  15. Merck 1996. Microbiology manual culture media. Editorial Merk 405pp.

  16. Probac do Brasil 1998. Meios para identificação de enteropatógenos. En Soros para identificação bacteriana. Editorial Probac do Brasil 75pp.

  17. National Committee for Clinical Laboratory Standards 1990. Performance standards for antimicrobiotic disc susceptibility test. 10 (7): 35-49.

  18. Bauer A.W., M.M. Kirby, J.L. Sherris and M. Turck 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45: 493-496.

  19. Leite C.Q.F., S.R. Valentini e D.P. Falcão 1988. Pesquisa de enteropatógenos em alimentos cárneos crus.Ci. Tecnol. Aliment. Vol. 8 No. 2: 115-227.

  20. Costa, F.N., L.M.C. Alves e S.S. Monte. 2000. Avaliação das condições higiênico-sanitárias de carne bovina moída, comercializada na cidade de São Luís, MA. Hig. Aliment. Vol.14 No. 77: 49-52.

  21. Franco, B.D.G.M e M. Landgraf., Op. cit.

  22. Brasil. Op. cit.

  23. Cerqueira A.M.F., A. Tibana and B.E.C. Guth 1997. High occurrence of Shiga-Like toxin producing strains among diarrheagenic Escherichia coli isolated from raw beef products in Rio de Janeiro city, Brazil. J. Food Prot. 60 (2): 177-180.

  24. Trabulsi, L.R. 1999. Bactéria encontrada no hambúrguer pode ser mortal. Editorial Agência USP de Notícias. 389: 99-101.

  25. Cerqueira A.M.F., et al, Op.cit.

  26. Franco, R.M. 2002. Escherichia coli: ocorrência em suínos abatidos na Grande Rio e sua viabilidade experimental em lingüiça frescal tipo Toscana. Tese de Doutorado em Medicina Veterinária – Universidade Federal Fluminense 153 pp.

  27. Hobbs, B.C. e D. Roberts., Op.cit.

  28. Franco, B.D.G.M e M. Landgraf., Op. cit.

  29. Onoue, Y., H. Konuma, H. Nakagawa, Y. Hara-Kudo, T. Fujita and S. Kumagai 1999. Collaborative evaluation of detection methods for Escherichia coli O157:H7 from radish sprouts and ground beef. Int. J. Food Microbiol. 46: 27-36

  30. Zhao T., M.P. Doyle, M.C. Kemp, R.S. Howell and P. Zhao 2004. Influence of freezing and freezing plus acidic calcium sulfate and lactic acid addition on thermal inactivation of Escherichia coli O157:H7 in ground beef. J. Food Prot. 67(8): 1760-1764.

  31. Silva N., V.C.A. Junqueira e N.F.A. Silveira 1997. Manual de métodos de análise microbiológica de alimentos. Editorial Varela 295pp.

  32. Onoue, Y., et al, Op.cit.

  33. Chirinos R.R.O., D.M. Vizeu, M.T. Destro, B.D.G.M. Franco and M. Landgraf 2002. Inactivation of Escherichia coli O157:H7 in hamburgers by gamma irradiation. Braz. J. Microbiol. 33: 53-56.

  34. Zhao T., et al, Op.cit.

  35. Petri C.M., L.A.F. Antunes e H.O. Saridakis 1989. Escherichia coli em produtos cárneos comercializados em Londrina- PR. Freqüência de Escherichia coli Enteropatogênica Clássica (EPEC). Rev. Microbiol. Vol.20 No. 4: 421-426.

  36. Meng J., S. Zhao, M.P. Doyle and S.W. Joseph 1998. Antibiotic resistance of Escherichia coli O157:H7 and O157:NM isolated from animals, food, and humans. J. Food Prot. 61 (11): 1511-1514.

  37. Oliveira L.A.T., T. Ferreira, R.M. Franco, e J.C.A.P. Carvalho 1999. Enumeração de Escherichia coli e Enterococcus em amostras de hambúrguer de frango, comercializadas em Niterói/RJ. Avaliação da sensibilidade a antimicrobianos das cepas isoladas. Hig. Aliment. Vol.13 No. 63: 49-55.

  38. Schroeder C.M., C. Zhao, C. DebRoy, J. Torcolini, S. Zhao, D.G. White, D.D. Wagner, P.F. Mc Dermott, R.D. Walker and J. Meng 2002. Antimicrobial resistance of Escherichia coli O157 isolated from humans, cattle, swine, and food. Appl. Environ. Microbiol. 68 (2): 576-581.

  39. Franco, R.M. Op.cit.

  40. Trabulsi, L.R. e M.R.F. Toledo 1989. Resistência bacteriana a droga. En Microbiologia [L.R. Trabulsi] Ed. Atheneu, Cap. 13: 86-89.

  41. Franco, R.M. Op.cit.

  42. Schroeder C.M., et al, Op.cit.

  43. Brasil 2001. Agência Nacional de Vigilância Sanitária. Resolução RDC n. 12, de 2 de janeiro de 2001. Diário Oficial da União, Brasília.




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