Guruvayoorappan C, Sudha G

Language: English
References: 39
Page: 358-363
PDF size: 110.37 Kb.

Text Extraction

Many anti-diabetic herbal preparations have been recommended in alternative systems of medicine for the treatment of diabetes. No systematic study has been done on the anti-diabetic efficacy of Byesukar, a polyherbal formulation to treat diabetes. The anti-diabetic efficacy of byesukar ethanol extract was evaluated in an animal model of diabetes induced by alloxan. Male Wistar rats were divided in to four groups. Group 1 was normal control group; group 2 and 3 received alloxan. After inducing experimental diabetes group 2 served as diabetic control; group 3 received byesukar (500 mg/kg body weight) orally for 30 consecutive days. Group 4 were normal rats which received byesukar extract alone. The effect of byesukar on glucose level in diabetic rats was studied and the level of glucose metabolizing enzymes (Hexokinase, glucose-6-phosphatase and fructose 1, 6-bisphosphatase) in the liver and kidney were estimated. The effect of byesukar on the serum and tissue lipid profile (Cholesterol, triglycerides, phospholipids and free fatty acids) were also estimated in diabetic rats. Our results indicate that treatment with byesukar resulted in significant reduction of blood glucose, tissue glucose-6-phosphatase and fructose 1, 6- bisphosphatase activity. The decreased tissue hexokinase activity in diabetes state was found to be significantly increased by byesukar treatment. Also the byesukar treated diabetic rats showed a significant decrease in the tissue lipid profile compared to the diabetic rats. In conclusion the decreased blood glucose accompanied with decreased lipid profile and changes in the activities of the glucose metabolizing enzymes shows the antidiabetic effect of byesukar.

REFERENCES

1. Scheen JA. Drug treatment of non-insulin dependent diabetes mellitus in the 1990s. Achievements and future development. Drug 1997; 54: 355-368.

2. Seifter S, England S. Energy metabolism, In: Arias I, Popper H, Schacter D, et al. (Eds). The Liver: Biology and Pathobiology. Rauen Press, New York. 1982; 219-249.

3. Sochor M, Baquer NZ, McLean P. Glucose over and under utilization in diabetes: Comparative studies on the change in activities of enzymes of glucose metabolism in rat kidney and liver. Mol Physiol 1985: 51-68.

4. Baquer NZ. Glucose over utilization and under utilization in diabetes and effects of antidiabetic compounds. Ann Real Acad Farm 1998; 64: 147-180.

5. Tiwari AK, Madhusudana Rao J. Diabetes mellitus and multiple therapeutic approaches of phytochemicals: Present status and future prospects. Curr Sci 2002; 83: 30-38.

6. Bhattaram VA, Ceraefe M, Kohlest C, Vest M, Deundorf H. Pharmacokinetics and bioavailability of herbal medicinal products. Phytomed 2002; 9: 1-36.

7. Bailey CJ, Day C. Traditional treatments for diabetes. Diabetes Care 1989; 12: 553-564.

8. Mitra SK, Gopumadhavan S, Muralidhar TS, Anturlikar SD, Sujatha MB. Effect of a herbomineral preparation D-400 in streptozotocin induced diabetic rats. J Ethnopharmacol 1996; 54: 41-46.

9. Bhattacharya SK, Satyan KS, Chakrbarti A. Effect of Trasina, an Ayurvedic herbal formulation, on pancreatic islet superoxide dismutase activity in hyperglycaemic rats. Indian J Exp Biol 1997; 35: 297-299.

10. Annapurna A, Kanaka, Mahalakshmi D, Murali Krishna K. Antidiabetic activity of a polyherbal preparation (tincture of punchparna) in normal and diabetic rats. Indian J Exp Biol 2001; 39: 500-502.

11. The WHO Expert Committee on diabetes mellitus. Technical report series 646. World Health Organization, Geneva 1980: 61.

12. Pari L, Ramakrishnan R, Venkateswarn S. Antihyperglycaemic effect of Diamed, an herbal formulation in experimental diabetes in rats. J Pharm Pharmacol 2001; 53: 1139-1143.

13. Pari L, Saravanan G. Antidiabetic effect of coagent db, a herbal drug in alloxan induced diabetes mellitus. Comp Biochem Physiol Part C 2002; 131: 19-25.

14. Subash Babu P, Prince PSM. Antihyperglycemic and antioxidant effect of hyponidd an ayurvedic herbomineral formulation in streptozotocin induced diabetic rats. J Pharm Pharmacol 2004; 56: 1435-1442.

15. Saravanan R, Pari L. Antihyperlipidemic and antiperoxidative effect of Diasulin, a polyherbal formulation in alloxan induced hyperglycemic rats. BMC Complement Altern Med 2005; 5: 14.

16. Guruvayoorappan C, Sudha G. Antioxidant potential of Byesukar, a polyherbal formulation on alloxan induced oxidative stress in rats. Malaysian J Biochem Mol Biol 2005; 11: 31-35.

17. Folch J, Lees M, Solane SGH. A simple method for isolation and purification of total lipids from animal tissues. J Biol Chem 1957; 226: 497-509.

18. Zlatkis A, Zak B, Boyle GJ. A method for the determination of serum cholesterol. J Clin Med 1953; 41: 486-492.

19. Foster JB, Dunn RT. Stable reagents for determination of serum triglycerides by colorimetric condensation method. Clin Chim Acta 1973; 19: 338-340.

20. Falholt K, Falholt W, Lund B. An easy colorimetric method for routine determination of free fatty acids in plasma. Clin Chim Acta 1973; 46: 105-111.

21. Zilversmit BB, Davis AK. Microdetermination of plasma phospholipids by TCA precipitation. J Lab Clin Med 1950; 35: 155-161.

22. Brandstrup N, Kirk JE, Bruni C. Determination of hexokinase in tissues. J Gerontol 1957; 12: 166-171.

23. Koida H, Oda T. Pathological occurrence of glucose-6-phosphatase in liver disease. Clin Chem Acta 1959; 4: 554-561.

24. Gancedo JM, Gancedo C. Fructose-1, 6-bis-phosphatase, phosphofructokinase and glucose-6-phosphate dehydrogenase from fermenting yeast. Arch Microbiol 1971; 76: 132-138.

25. Milgro FI, Martinez JA. Effect of the oral administration of a beta 3-adrenergic agonist on lipid metabolism in alloxan-diabetic rats. J Pharm Pharmacol 2000; 52: 851-856.

26. Rajagopal K, Sasikala K. Antihyperglycaemic and antihyperlipidaemic effects of Nymphaea stellata in alloxan-induced diabetic rats. Singapore Med J 2008; 49(2): 137-141.

27. Pari L, Maheswari JU. Hypoglycaemic effect of Musa sapientum L. in alloxan-induced diabetic rats. J Ethnopharmacol 1999; 68: 321-325.

28. Gupta BL, Nehal M, Baquer NZ. Effect of experimental diabetes on the activities of hexokinase, glucose-6-phosphate dehydrogenase and catecholamines in rat erythrocytes of different ages. Indian J Exp Biol 1997; 35: 792-795.

29. Seifter S, England S. Energy metabolism. In: The Liver: Biology and pathology. Arias I, Papper M, Schacter D (Ed.) Raven Press, New York 1982; 219-249.

30. Baquer NZ. Glucose over utilization and under utilization in diabetes and and effects of antidiabetic compounds. Ann Renal Acad Farm 1998; 64: 147-180.

31. Laakso M, Malkki M, Deeb SS. Amino acid substituents in hexokinase II among patients with NIDDM. Diabetes 1995; 44: 330-334.

32. Murray RK, Granner DK, Mayes PA, Rodwell VW. Harper’s Biochemistry. 25th Ed. Stamford, Connecticut: Appleton and Lange. 2000: 610-617.

33. Sharma SR, Dwivedi SK, Swarup D. Hypoglycemic and hypolipidaemic effects of Cinnamomum tomala nees leaves. Indian J Exp Biol 1996; 34: 372-374.

34. Luo Q, Cai Y, Yan J, Sun M, Corke H. Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life Sci 2004; 76: 137-149.

35. Goodman LS, Gilman A. The pharmacological basis of therapeutics. 7th Edition. Mac Millan, New York 1985; 1490-1510.

36. Bopanna KN, Kannan J, Sushma G, Balaraman R, Rathod SP. Antidiabetic and antihyperlipaemic effects of neem seed kernel powder on alloxan diabetic rabbits. Indian J Pharmacol 1997; 29: 162-167.

37. Nageswara RG, Mahesh KP, Dhandapani VS, Rama KT, Hayashi T. Constituents of Cassia auriculata. Fitoterapia 2000; 71(1): 82-83.

38. Timbola AK, Szpoganicz B, Branco A, Monache FD, Pizzolatti MG. A new flavanol from leaves of Eugenia jambolana. Fitoterapia 2002; 73(2): 174-176.

39. Boonsri S, Karalai C, Ponglimanont C, Chantrapromma S, Kanjana-Opas A. Cytotoxic and antibacterial sesquiterpenes from Thespesia populnea. J Nat Prod 2008; 71(7): 1173-1177.