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Asociación Mexicana de Gastroenterología

2007, Number 1

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Rev Gastroenterol Mex 2007; 72 (1)

Efficacy of absorbance ratio of ELISA for hepatitis C virus of 3th generation in the prediction of viremia evaluated by PCR

Vázquez-Ávila I, Vera-Peralta JM, Álvarez-Nemegyei J, Rodríguez-Carvajal O

Language: Spanish
References: 15
Page: 34-39
PDF size: 48.92 Kb.


ABSTRACT

Background: In order to decrease the burden of suffering and the costs derived from confirmatory molecular assays, a better strategy is badly needed to decrease the rate of false positive results of the enzyme-linked immunoassay (ELISA) for detection of hepatitis C virus (HCV) antibodies (Anti). Objective: To establish the best cutoff of the S/CO rate in subjects with a positive result of a microparticule, third generation ELISA assay for Anti-HCV, for predicting viremia as detected by polymerase chain reaction (PCR) assay. Methods: Using the result of the PCR assay as “gold standard”, a ROC curve was build with the results of the S/CO rate values in subjects with a positive result for ELISA HCV assay. Results: Fifty two subjects (30 male, 22 female, 40 ± 12.5 years old) were included. Thirty four (65.3%) had a positive RNA HCV PCR assay. The area under the curve was 0.99 (95% CI: 0.98-1.0). The optimal cutoff for the S/CO rate was established in 29: sensitivity: 97%; specificity: 100%: PPV: 100%; NPV: 94%. Conclusions: Setting the cutoff of the S/CO in 29 results in a high predictive value for viremia as detected by PCR in subjects with a positive ELISA HVC assay. This knowledge may result in a better decision taking for the clinical follow up of those subjects with a positive result in the ELISA screening assay for HCV infection.


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