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Anales de la Sociedad Mexicana de Oftalmología y Archivos de la Asociación Para Evitar la Ceguera en México

2008, Number 4

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Rev Mex Oftalmol 2008; 82 (4)

Efecto en corteza cerebral por interrupción de binocularidad en ratas. Análisis de neurotransmisores por cromatografía líquida de alta resolución

Salas-Cervantes MR, Moguel-Ancheita S, Etulain-González A, Figueroa-Barcob S, Glennie-Graue G, Palomera M

Language: Spanish
References: 19
Page: 231-241
PDF size: 77.53 Kb.


ABSTRACT

Objectives: To identify which changes are present at the brain cortex after the interruption of binocular vision. To identify which is the most altered neurotransmitters and relate the behavioral changes with the neural adaptation after the lack of binocular vision.
Material and methods: A prospective, transversal, experimental and comparative study was designed, from September 25th 2004 to March 1st 2005. Thirty, 1-month old Wistar rats were used, 15 females and 15 males of 80 to 100 gr. All animals were placed into acrylic cages, with free access to food and water (nutri-cubo, Purina, USA), the animals were divided into 5 experimental groups: Group 1: Control rats (n=6); Group 2: rats with 72 hours of unilateral eye occlusion (n=6); Group 3: rats with 1 week of unilateral eye occlusion (n=6); Group 4: rats with 2 weeks of unilateral eye occlusion (n=6); Group 5: rats with 3 weeks unilateral eye occlusion (n=6). The interruption of binocular vision was achieved by performing a unilateral right eye tarsorrhaphy with 6-0s silk under ether sedation. Before and after the eye occlusion a memory prevention passive test of short and long terms was performed. All animals were scarified and catecholamine were measured by HPLC (High resolution liquid chromatography)
Results: The results showed alteration in the levels of neurotransmitters and memory in response to the changes of binocular vision.
Conclusions: A failure of binocular vision, provoke a deficit in the cortical sensor-system. This is evident in the behavior, learning and memory presented in the animals of this study in relation with the amount of neurotransmitters reported at different levels of the Central Nervous System.


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