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Anales de la Sociedad Mexicana de Oftalmología y Archivos de la Asociación Para Evitar la Ceguera en México

2007, Number 2

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Rev Mex Oftalmol 2007; 81 (2)

Angiogénesis retiniana inducida por oxígeno en ratón: un modelo económico

Parra-Colín P, Domínguez-Dueñas F, Gracia-Mora MI, Rodríguez-Reyes A, Quiroz-Mercado H

Language: Spanish
References: 19
Page: 106-109
PDF size: 110.55 Kb.


ABSTRACT

Purpose: To reproduce a cheap model of oxygen-induced retinopathy in mouse in which the retinal neovascularization could be reproducible and quantifiable to further study the pathogenesis and interventional therapy of proliferative retinopathies
Methods: Neonatal mice C57BL/6:Hsd from UNAM-Harlan Centre were exposed at 7 days postnatal to 75% oxygen during 5 days. At day 12 postnatal they were returned to normoxic conditions and were killed 8 days later. After enucleation, the eyes were histologically analyzed and the endothelial cells in the retina were counted.
Results: In the mice exposed to hyperoxia the average endothelial cell nuclei were 175.3 ± 15.9 SD, compared to the nonexposed with an average of 58.2 ± 7.5. We used the Mann-Whitney non-parametric statistical test with a p=0.002.
Conclusions: We achieved to reproduce an oxygen-induced retinopathy model using less expensive resources.


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