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Anales de la Sociedad Mexicana de Oftalmología y Archivos de la Asociación Para Evitar la Ceguera en México

2007, Number 1

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Rev Mex Oftalmol 2007; 81 (1)

Valoración de la respuesta inmunológica sistémica en pacientes con queratitis herpética

Aguilar-Velázquez G, Santacruz-Valdés C, Chávez R, Estrada-García I, Estrada-Parra S, Jiménez-Martínez MC

Language: Spanish
References: 24
Page: 7-13
PDF size: 545.55 Kb.


ABSTRACT

Introduction: T cells play an important role in the immunepathogenesis of herpetic stromal keratitis (HSK). It has been demonstrated, on animal models, that T cells infiltrate the cornea and produce large quantities of IFN-γ. In contrast, the immune response in humans has not been broadly studied.
Aim: To evaluate cytokine profile on peripheral blood mononuclear cells (PBMC) from HSK patients (p).
Methods: We determined on PBMC from HSK-p and healthy controls (HC) the expression of CD4, CD8, intracellular cytokines (IFN-γ/IL-4) and proinflammatory cytokines (TNF-α/IL-6/IL-1b) in culture supernatants (SN) before/after polyclonal stimuli. Results were obtained by flow-cytometry and ELISA.
Results: Percentage (%) of CD4+IFN-γ+ T cells in HSK-p 16 vs 8 in HC (p=0.024); % of CD4+IL-4+ T cells in HSK-p 7 vs 4 in HC; % of CD8+IFN-γ+ T cells in HSK-p 33 vs 31 in HC; % of CD8+IL-4+ T cells in HSK-p 5 vs 6 in HC; % of CD4+IL 4+ T cells 7 vs 16 CD4+IFN-γ + T cells from HSK-p (p=0.006). Cytokines in SN (pg/ml): TNF-α in non stimulated cells (NSC) from HSKp 285 vs 3740 in stimulated cells (SC) from HSK-p (p‹0.001); TNF-α in NSC from HC 172 vs 3364 in SC from HC (p‹0.001); IL-1β in NSC from HSK-p 53 vs 107 in SC from HSK-p (p=0.038); IL-1β in NSC from HC 37 vs 54 in SC from HC; IL-1β in SC from HSK-p 107 vs 54 in SC from HC (p=0.029); IL-6 in NSC from HSK-p 328 vs 481 in SC from HSK-p (p=0.026).
Conclusions: Stimulated T cells from HSK-p were predominantly IFN-γ+. It is well known that IFN-γ induces production of proinflammatory cytokines from activated macrophages, in our study we find IL-1b as the most important proinflammatory cytokine increased after in vitro stimuli in patients with herpetic stromal keratitis. These results suggest that peripheral IFN-γ+ cells could migrate to the human cornea and to collaborate with residents or infiltrating cells to produce proinflammatory cytokines, increasing the inflammatory damage as it has been demonstrated in animal models.


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