2008, Number 2
VI. La biología molecular en el diagnóstico micológico en México
Hernández-Hernández F
Language: Spanish
References: 8
Page: 134-136
PDF size: 35.72 Kb.
ABSTRACT
In the last 20 years the molecular biology has reached all the medicine areas. The medical mycology has not been the exception and one of its major objectives is the early identification of the etiological agents of fungal infections. The polymerase chain reaction (PCR) has been the molecular technique most widely used. However in Mexico the development of these procedures is yet limited mainly because it requires highly specialized staff and the high costs. Therefore, the laboratories which have succeeded in the standarization of this technique and offer it as a part of the hospital services are very few. Infections which get more attention are systemic mycosis caused by low virulence fungi such as Candida, Aspergillus, Cryptococcus, Pneumocystis and some mucorales species, due to the high mortality in patients with severe risk factors. Some of these species are difficult to identify by non molecular procedures such as culture and histopathology which are time consuming, so the establishment of these methods in the mycological diagnosis laboratories is necessary.REFERENCES
Baires-Varguez L, Cruz-García A, Villa-Tanaka L, Sánchez-García S, Gaitán-Cepeda LA, Sánchez-Vargas LO, et al. Comparison of a randomly amplified polymorphic DNA (RAPD) analysis and ATB ID 32C system for identification of clinical isolates of different Candida species. Rev Iberoam Micol 2007;24:148-151.