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2008, Number 2

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Rev Mex Patol Clin Med Lab 2008; 55 (2)

Nitric oxide and malondialdehyde serum levels in subjects with positive IgM for herpes simplex virus

Gutiérrez-Salinas J, Cruz-Tovar L

Language: Spanish
References: 42
Page: 111-119
PDF size: 131.14 Kb.


ABSTRACT

Background: Herpes simplex virus (HSV) infection it can produced is able give rise to diverse metabolic alterations in the human being. In vitro and in vivo experimental research shows that both, oxidative and nitrosative stress are developed by HSV infection. However, the latter is not known in humans that present an infection with this virus. Objective: The aim of this study was to determine both malondialdehyde (MDA) and total nitrates (NOx) as indicative of an oxidative stress and nitrosative stress, in patients with positive IgM for HSV virus. Material and methods: Eighteen serum samples from subjects with an IgM positive for HSV were analyzed. As control, serum from 20 normal subjects HSV negative was used. Both MDA and NOx were analyzed in all serum samples by spectrophotometric techniques. Results: The mean ± SEM of IgM index in serum of subjects with HSV was 1.767 ± 0.748. The MDA concentration was highly in the subjects infected with HSV as compared with controls (2.337 ± 0.4676 vs 0.5773 ± 0.0301, p ‹ 0.05 respectively). By the same way, NOx concentration was greater in HSV-infected subjects as compared with controls (76.32 ± 6.206 vs 37.04 ± 1.073; p ‹ 0.05, respectively). A significant positive correlation between IgM index and MDA and IgM index and NOx was show in HSV-infected subjects. Conclusion: The IgM-positive for HSV have an increase in both MDA and NOx metabolites indicative of a oxidative and nitrosative stress.


REFERENCES

  1. Corey L, Spear PG. Infections with herpes simplex viruses. N Engl J Med 1986; 314: 686-691.

  2. Timbury MC, Edmond E. Herpesviruses. J Clin Pathol 1979; 32: 859-881.

  3. DeBolle L, Naesens L, DeClercq E. Update on human herpesvirus 6 biology, clinical features, and therapy. Clin Microl Rev 2005; 18: 217-245.

  4. Smith JS, Robinson J. Age-specific prevalence of infection with herpes simplex virus types 2 and 1: A global review. J Infect Dis 2002; 186 (Suppl 1): S3-S28.

  5. Koelle DM, Corey L. Recent progress in herpes simplex virus immunobiology and vaccine research. Clin Microbiol Rev 2003; 16: 96-113.

  6. Koelle DM, Corey L. Herpes simplex: Insights on pathogenesis and possible vaccines. Annu Rev Med 2008; 59: 381-395.

  7. Johnson RE, Nahmias AJ, Magdar LS, Lee CA. A seroepidemiologic survey of the prevalence of herpes simplex virus type 2 infection in the United States. N Engl J Med 1990; 321: 7-12.

  8. Conde-Gonzalez CJ, Juarez-Figueroa L, Uribe-Salas F, Hernandez-Nevarez P, Schmid DS. Analysis of herpes simplex virus 1 and 2 infection in women with high risk sexual behaviour in México. Int J Epidemiol 1999; 28: 571-576.

  9. Conde-González CJ, Lazcano-Ponce E, Hernández-Girón C, Juárez-Figueroa L, Schmid JS. Seroprevalencia de la infección por el virus herpes simplex tipo 2 en tres grupos poblacionales de la ciudad de México. Sal Pub Mex 2003; 45 (supl) 5: S608-S616.

  10. Barba EJR. Citomegalovirus y trasplante renal: Una combinación peligrosa. Rev Mex Patol Clin 2006; 53: 52-61.

  11. Griffiths PD, Clark DA, Emery VC. Betaherpesvirus in transplant recipients. J Antimicrobiol Chemother 2000; 45: 29-34.

  12. Stamm WE, Handsfiel HH, Rompalo AM, Ashely RL, Roberts PL. Association between genital ulcer disease and acquisition of HIV infection in homosexual men. J Am Med Assoc 1988; 260: 1429-1433.

  13. Hook EW, Cannon RQ, Nahmias AJ, Lee FF. Herpes simplex virus infection as a risk factor for HIV infection in heterosexuals. J Infect Dis 1992; 165: 251-255.

  14. Shoshces RC, Komatsu T. Does nitric oxide play a critical role in viral infections? J Virol 1998; 72: 4547-4551.

  15. Hasan-Zaki M, Akuta T, Akaike T. Nitric oxide-induced nitrative stress involved in microbial pathogenesis. J Pharmacol Sci 2005; 98: 117-129.

  16. Elbim C, Pillet S, Prevost MH, Preira A, Girard PM. Redox and activation status of monocytes from human immunodeficiency virus-infected patients: relationship with viral load. J Virol 1999; 73: 4561-4566.

  17. Alland JP, Afhdassi E, Chau E, Salit I, Walmsley S. Oxidative stress and plasma antioxidant micronutrients in humans with HIV infection. Am J Clin Nutr 1998; 67: 143-147.

  18. Jiang Y, Scofiel VL,l Yan M, Qiang W, Liu N, Reid AL. Retrovirus-induced oxidative stress with neuroimmunodegeneration is suppressed by antioxidant treatment with a refined monosodium alpha-lumino (Galavit). J Virol 2006; 80: 4557-4569.

  19. Gutiérrez-Salinas J, Morales-González JA. La ingesta de fluoruro de sodio produce estrés oxidativo en la mucosa bucal de la rata. Rev Mex Cien Farm 2006; 37: 11-22.

  20. Gutiérrez-Salinas J, Morales-González JA. Producción de radicales libres derivados del oxígeno y el daño al hepatocito. Med Int Mex 2004; 20: 287-295.

  21. Gutiérrez-Salinas J. ¿Qué sabe usted acerca de radicales libres? Rev Mex Cien Farm 2006; 37: 69-73.

  22. Halliwell B, Gutteridge JMC. Oxygen free radicals and iron in relation to biology and medicine: Some problems and concepts. Arch Biochem Biophys 1986; 246: 501-514.

  23. Nielsen F, Mikkelsen BB, Nielsen JB, Andersen HR, Grandjean P. Plasma malondialdehyde as biomarker for oxidative stress: Reference interval and effects of life-style factors. Clin Chem 1997; 43: 1209-1214.

  24. Toda N, Okamura T. The pharmacology of nitric oxide in the peripheral nervous system of blood vessels. Pharmacol Rev 2003; 55: 271-324.

  25. Tarpey MM, Fridovich I. Methods off detection of vascular reactive species. Nitric oxide, superoxide, hydrogen peroxide and peroxynitrite. Circ Res 2001; 89: 224-236.

  26. Dalle-Donne I, Rossi R, Colombo R, Giustarini D, Milzani A. Biomarkers of oxidative damage in human disease. Clin Chem 2006; 52: 601-623.

  27. Sheridan PA, Beck MA. The immune response to herpes simplex virus encephalitis in mice is modulated by dietary vitamin E. J Nutr 2008; 138: 130-137.

  28. Palú G, Biasolo MA, Sartor G, Masotti L, Papini E. Effects of herpes simplex virus type 1 infection on the plasma membrane and related functions of HeLa S-3 cells. J Gen Virol 1994; 75: 3337-3344.

  29. Marin J, Kese D, Potocnik M, Butina R. Laboratory diagnosis of herpesviruses. Acta Dermatovenerol APA 2000; 9: 1-7.

  30. Dobec M, Bossart W, Kaeppeli F, Mueller-Schoop J. Serology and serum DNA detection in shingles. Swiss Med Wkly 2008; 138: 47-51.

  31. Quinlivan M, Breuer J. Molecular studies of varicella zoster virus. Rev Med Virol 2006; 16: 225-250.

  32. Dickerson FB, Boronow JJ, Stalling C, Origoni AE, Ruslanova I, Yolken RH. Association of serum antibodies to herpes simplex virus 1 with cognitive deficits in individuals with schizophrenia. Arch Gen Psychiatry 2003; 60: 466-472.

  33. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 351-358.

  34. Ramírez-Farías C, Madrigal-Santillán E, Gutiérrez-Salinas J, Rodríguez-Sánchez N, Martínez-Cruz M, Valle-Jones I et al. Protective effect of some vitamins against the toxic action of ethanol on liver regeneration induced by partial hepatectomy in rats. World J Gastroenterol 2008; 14: 899-907.

  35. Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by kinetic cadmium-reduction method. Clin Chem 1990; 38: 1440-1443.

  36. Gutiérrez-Salinas J, Cruz-Tovar L, García-Méndez S. Incremento en la concentración de óxido nítrico y meta-hemoglobina en eritrocitos contenidos en bolsas para transfusión sanguínea. Rev Mex Patol Clin 2008; 55: 21-28.

  37. von Müller L, Klemm A, Weiss M, Shneider M. Active cytomegalovirus infection in patients with septic shock. Emerg Infect Dis 2006; 12: 1517-1522.

  38. Muller F. Reactive oxygen intermediates and human immunodeficiency virus (HIV) infection. Fre Rad Biol Med 1992; 13: 651-657.

  39. Maeda H, Akaike T. Oxygen free radicals as pathogenic molecules in viral diseases. Proc Soc Exp Biol Med 1991; 198: 721-727.

  40. Sanders SP, Siekiersi ES, Portes JD, Richards SM, Proud D. Nitric oxide inhibits rhinovirus-induced cytokine production and viral replication in a human respiratory epithelial cell line. J Virol 1998; 72: 934-942.

  41. Akaike T, Fujii S, Kato A, Yoshitake J, Miyamoto Y, Sawa T. Viral mutation accelerated by nitric oxide production during infection in vitro. FASEB J 2000; 4: 1447-1454.

  42. Peterhans E. Oxidants and antioxidants in viral diseases: Mechanisms and metabolic regulations. J Nutr 1997; 127: 962S-965S




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