Arenas QR, Saucedo OJA, López PSR

Language: Spanish
References: 15
Page: 150-154
PDF size: 463.41 Kb.

ABSTRACT

The following paper reports on a study of tissue regeneration using Plasma Rich in Growth Factors (PRGF) on random skin flaps. Viability, effects on cellularity and neovascularity are determined by histological analysis. Dermal flaps with ratio 1:5 were made cut into the dorsal skin of 16 Guinea pigs divided into 2 groups of 8. PRGF was applied randomly on the bed of the flap on one group, while saline solution (SS) was applied to the other group. 14 days later the viability of the flaps was determined clinically and then resected and studied under the microscope. The PRGF group exhibited greater percentage of viability (69%) versus the SS group (41%) with a significant p = 0.04. The PRGF group presented a significant decrease in macrophages (p = 0.03), lymphocytes (p = 0.001), plasma cells (p = 0.02), as well as a significant increase in fibroblasts (p = 0.001) and collagen (p = 0.038), which did not occur in the SS group. No significant changes in inflammation or neovascularization were observed. The present study demonstrates that PRGF improves the survival of random skin flaps, as well as the migration of fibroblasts and the production of collagen.

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