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1998, Number 4

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Vet Mex 1998; 29 (4)

Effect of equine follicular fluid and bovine somatotropin in the survival of ovine embryos asynchronously transferred

Mejía VO, Zarco QLA, Rojas MS, Rosas GM, Valencia MJ

Language: English/Spanish
References: 43
Page: 359-367
PDF size: 953.71 Kb.


ABSTRACT

Maternal-embrionary asynchrony could be overcome by increasing progesterone (P4) levels to improve embryo development or by delaying luteolisis. With this aim, asynchronous recipients were treated with bovine somatotropin (ST) or equine follicular fluid (EFF). Six day embryos were collected from 25 donors. Estrous was synchronized with fluorogestone acetate and PGF2α. Progestagen was removed in asynchronous recipients 3.5 days before the donors. Recipients were alocated to 5 groups: synchronous (syn), n= 15; asynchronous (asyn), n= 16; asynchronous + EFF (asyneff), n= 19; asynchronous + ST (asynst), n=7 and asynchronous + EFF + ST (asynffst), n= 10. Progesterone levels were higher (3.3 ng/ml) in asyneff recipients (P‹0.05), and the lowest levels (1.4 ng/ml) were found in the asynst ones. Luteal phase was longer (16.8 days) in the non pregnant ewes of the asyn group. Luteolisis was delayed in 4 ewes of the asyneff until day 19. Pregnancy rate was not different between the syn and asyn groups (86.6 vs. 68.7%). Treatment with EFF and ST did not improve pregnancy rates in asynchronous recipients even when P4 levels were increased in pregnant asyneff and asynffst recipients or when luteolisis was delayed in the asyneff ones.


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