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1999, Number 4

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Vet Mex 1999; 30 (4)

Distribution of mast cells in mammary gland stroma, during the active and inactive periods in the bitch (Canis familiaris)

Villaseñor GH, Emilia LR, Anzaldúa ASR, Pérez MM

Language: English/Spanish
References: 40
Page: 317-321
PDF size: 86.75 Kb.


ABSTRACT

Morphologic characteristics of stroma and parenchima depend of the functional level in the mammary gland. Mast cells (MC) are dynamic elements present in connective tissues, which can induce degranulation of biologically active mediators which the interaction with other connective cells. The objective of this study was to find out the number and pattern of distribution and possible differences in mast cell population present in the mammary gland (MG) of bitches under two physiologic conditions: active or inactive. Forty adult bitches from the Antirrabic Health Center were sacrificed and sampled. Fragments from abdominal, cranial and caudal mammary gland were collected, processed and included in paraffin wax, cut in thin 6 µm sections and stained with hematoxiline-eosine and tolouidine blue. The total number of MC was counted in 16 selected fields at random to estimate the mm2 population from both interalveolar and interlobulillar stroma. Statistical analysis showed significant differences (P‹0.05) between the interalveolar (R1) and interlobulillar regions (R2) from inactive MG. Differences were found in MC and between inactive MG and R1 and R2 from active MG. During the active period the number of MC were significantly decreased in relation to that one during the inactive period; this suggests that MC play a relevant role in proliferative changes and growth from interalveolar connective cells of the inactive MG. In this period, the connective tissue includes all the space from the glandular epithelium present in the active period.


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