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1999, Number 3

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Vet Mex 1999; 30 (3)

Motility and fertility of frozen ram semen thawed in two different rhythms of temperature

Angulo MRB, Ortíz HA, Berruecos VJM, Feldman SD, Valencia MJ

Language: English/Spanish
References: 22
Page: 265-268
PDF size: 37.96 Kb.


ABSTRACT

The aim of the study was to compare the effect of two temperatures and thawing rates on motility and fertility of frozen ram semen. A total of 208 ewes and 13 rams of different breeds were used. Semen was collected with an artificial vagina, and it was evaluated, diluted with a Tris-citric acid-fructose-glicerol-yolk extender and centrifugated at 200g/15 min. The sediment was then resuspended with the same extender. Diluted semen was frozen in 0.25 ml straws containing 300 millions of motile sperm. Post-thaw motility was evaluated in 212 straws which were thawed in a water bath at 36°C/8 sec (Group 1) or 70°C/4 sec (Group 2), and another 208 straws were used for intracervical insemination of ewes. Ewes lambed (%) per ewes inseminated with semen thawed at different rates was also evaluated. Post-thaw motility was 51.28 and 47.98% for Groups 1 and 2, respectively, and fertility was 30.7 and 29.2% for the same groups, respectively, without differences between groups (X2; P›0.05). Thawing straws at 36°C/8 sec is a more practical and safer method.


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