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1999, Number 3

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Vet Mex 1999; 30 (3)

Isolation and identification of Mycobacterium bovis from, sputum samples of human patients with chronic respiratories diseases

Toledo OP, Milian SF, Santillan FMA, Ramírez CIC

Language: English/Spanish
References: 19
Page: 227-229
PDF size: 32.64 Kb.


ABSTRACT

In order to identify through microbiological methods the presence of mycobacteria in sputum samples of human patients with chronic respiratory problems, nine Ziehl-Neelsen (ZN) positive, and forty one ZN negative sputum samples from patients with chronic respiratory disease were included in the study. Each sample was cultured in both, Lowenstein-Jensen with glycerol and Stonebrink with pyruvate medium. After at least 8 weeks at 37°C, the nine ZN positive samples were also positive to culture, and from the forty one ZN negative group, ten (24%) were positive to culture. Thirteen out of all the cultures were identified as Mycobacterium tuberculosis, 3 as M. Bovis, and in 3 of the cultures, both M. tuberculosis and M. bovis were found in the same tube. The fact that 24% of the ZN negative group were culture positive, shows the importance of including mycobacteria culture in the routine diagnosis of tuberculosis. More than that, whenever possible, biochemical identification of species should be included, since patients infected with M. bovis will not respond appropriately to the pyrazinamide treatment.


REFERENCES

  1. Kantor NI, Álvarez E. Situación de la tuberculosis bovina en América Latina. Publicación especial No.10. Martínez, Argentina: Panamericano de Zoonosis, Organización Mundial de la Salud, 1990.

  2. Darbon CJ , Grange JM. HIV/AIDS and its implications for the control of animal tuberculosis. Br Vet J 1993;149:405-417.

  3. Barnes PF, Bloch AB, Davidson PT, Snider DE. Tuberculosis in patients with human immunodeficiency virus infection. N Engl J Med 1991; 324: 1644-1650.

  4. Centers of Disease Control. Tuberculosis and human immunodeficiency virus infection: recommendations of the advisory committee for the elimination of tuberculosis. Morb Mortal Wkly Rep 1989;38:236-238.

  5. Quinn TC. Interaction of the human immunodeficiency virus and tuberculosis and the implications for BCG vaccination. Rev Infect Dis 1989;11(Suppl 2):379-384.

  6. Reider HL, Cauthen GM, Kelly GD, Bloch AB, Snider DE. Tuberculosis in the United States. J Am Med Assoc 1989;262:385-389.

  7. Dankner WM, Waecker JN, Essey AM, Moser K, Thompson M, Davis ECh. Mycobacterium bovis infections in San Diego: a clinicoepidemiologic study of 73 patients and a historical review of a forgotten pathogen. Medicine 1993;72:11-37.

  8. Carter GR, Chengappa MM. Bacteriología y micología veterinarias. Aspectos esenciales. 2ª ed. México (DF): El Manual Moderno, 1994.

  9. Worthington RW, Kleeberg HH. Practical problems in tuberculin testing in cattle. J S Afr Vet Assoc 1965;37:177-183.

  10. Collins CH, Grange JM. A review: the bovine tubercle bacillus. J Appl Bacteriol 1983;55:13-29.

  11. Konno K, Feldman FM, McDermott W. Pyrazinamide susceptibility and amidase activity of tubercle bacillus. Am Rev Respir Dis 1967;95:461-469.

  12. Secretaría de Salud. NOM -006-55A2-1993 para la prevención de la tuberculosis en la atención primaria de la salud. Diario Oficial de la Federación. México (DF): 26 de enero de 1995.

  13. Payeur JB, Jarnagin JL, Marquardt JG, Schaper AL, Martin, MB. Laboratory methods in veterinary mycobacteriology for the isolation and identification of mycobacteria. Ames (Ia): United States Department of Agriculture, 1993.

  14. Morilla GA, González VD. Introducción al diagnóstico inmunológico de las enfermedades de los animales domésticos. México (DF): Instituto Nacional de Investigaciones Pecuarias, Secretaría de Agricultura y Recursos Hidráulicos, 1998.

  15. Grange MJ, Yates DM. Zoonotic aspects of Mycobacterium bovis infection. Vet Microbiol 1994;40:137-151.

  16. Styblo K. Overview and epidemiologic assessment of the current global tuberculosis situation with an emphasis on control in developing countries. Rev Infect Dis 1989;2 (Suppl 2):339-346.

  17. Lumb R, Shaw D. Mycobacterium bovis (BCG) vaccination. Progressive disease in a patient asymptomatically infected with the human immunodeficiency virus. Austr Med J 1992;156:286-287.

  18. Smith E, Thybo S, Bennedsen J. Infection with Mycobacterium bovis in a patient with AIDS: a late complication of BCG vaccination. Scand J Infect Dis 1992;24:109-110.

  19. Von Reyn CR, Clements CJ, Man JM. Human immunodeficiency virus infection and routine childhood immunisation. Lancet 1987;2:669-672.




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