Canto AGJ, Figueroa MJV, Ramos AJA, Alvarez MJA, Mosqueda GJJ, Vega y MCA

Language: English/Spanish
References: 19
Page: 215-220
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ABSTRACT

The main objective of this work was to evaluate the inocuity and immunoprotection capability of a combined immunogen of Babesia bigemina and Babesia bovis at doses of 1x10⁹ and 1x10⁸, respectively, under a controlled challenge. In the first experiment (inocuity), 16 Holstein bovines from a free tick area, and negative to antibodies against Babesia spp by the indirect fluorescent test, were used. Animals were inoculated with 1x10⁹ infected red blood cells (RBC) of an attenuated strain of Babesia bigemina and 1x10⁸ RBC of a clone of Babesia bovis. Both derived from an in vitro culture. Physiological parameters were monitored from day 7 to day 14 post inoculation (PI), and minimal changes were observed. The second experiment (immunogenicity) was performed 3 months PI, and consisted in the challenge of 8 animals of the previous inoculated bovines from experiment 1 with virulent strains at a dose of 1x10⁸ RBC, of both species. Four additional animals were used as the control group. The immunized group presented a small drop in the packed cell volume (PCV). No changes in rectal temperature were observed, and the percentage of parasitised erythrocytes (PPE) was 0.06% and ‹0.01% for B. bigemina and B. bovis, respectively. In the control group a rectal temperature over 40°C was observed; the PCV dropped (29%), and the PPE was 0.5% for B. bigemina and 0.3% for B. bovis. These animals required treatment in order to prevent mortality. It is concluded, that the combined immunogen of B. bigemina and B. bovis at high doses does not produce severe clinical reactions in susceptible animals, and evokes a solid protection against virulent strain challenge.

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