Olazabal ME, Morales MA, Serrano PH, Brito AE

Language: English/Spanish
References: 28
Page: 109-115
PDF size: 49.48 Kb.

ABSTRACT

This study included two experiments: Trial I in which two methods (necropsy and polyethylene bags) to collect Fasciola hepatica metacercariae were compared, and Trial II carried out in order to determine the invasive power of the metacercariae obtained in an artificial biotope using Wistar rats and Balb/c mice challenged with 20 and 5-10 metacercariae, respectively. It was found that the number of metacercariae obtained through the use of polyethylene bags is considerably higher (p‹0.01) than the dissection (1442 metacercariae were obtained with a mean of 12.87 per host as opposed to a 29.31 mean for polyethylene bags with a total of 3313 metacercariae). Both invasive power (IP) and extent (IE) in rats was higher in the third week (IP, 23%; IE, 80%). The same happened in the mice challenged with infective doses of 10 metacercariae (IP, 23%; IE, 80%). There was a 100% damage in the liver for both doses in the third week. It may be concluded that the polyethylene bag method is more effective than the dissection one, since it makes it possible to collect a larger number of invasive forms with a higher quality, cleanliness and easy removal of the metacercariae from the encystment surface, as well as due to the fact that the invasive power of the metacercariae and the extent of the invasion obtained, allows their use in test models of Fasciola hepatica in rats and mice with the appropriate doses of 10 and 20 metacercariae for mice and rats, respectively.

REFERENCES

1. Babicek K, Danek J. Checking the method of testing antifasciolles on the model helminth Fasciola hepatica in experimentally invaded laboratory Wistar rats. Biol Chem Vet (Praha) 1989;25:311-320.

2. Jang DH, Young HJ, Jum GS. Study on the metacercarial productivity of Fasciola sp intermediate host Austropeplea ollula (Lymnaea ollula). Korean J Vet Res 1987;27:291-299.

3. Mauri-Maida, Mitterpak J, Brito E. Embriogonia de la Fasciola hepatica Linneo, 1758 en las condiciones de Cuba. Rev Cub Cienc Vet 1980;11:93-97.

4. Taira N, Yoshifuji H, Boray JC. Zoonotic potential of infection with Fasciola spp. By consumption freshly prepared raw liver containing immature flukes. Int J Parasitol 1997;27:775-779.

5. Ibarra OF, Jenkins DC. An in vitro screen for new fasciolicidal agents. Parasitenkunde 1984;70:665-661.

6. Boray JC, Happich FA, Andrews JC. Studies on the suitability of the albino rat for testing anthelmintic activity against Fasciola hepatica. Ann Trop Med Parasitol 1967;61:104-111.

7. Corba J. Studies on the development of Fasciola hepatica in rats. Folia Vet 1972;16:133-138.

8. Andrews P. The mouse as experimental host for Fasciola hepatica. Helminthologia 1979;16:207-215.

9. Corba J, Velebny S, Spaldonova R. Primary screening of newly synthetized substances in white mice invaded with Fasciola hepatica. Helminthologia 1981;18:43-52. with Fasciola hepatica. Helminthologia 1981;18:43-52.

10. Mitterpak J, Méndez M, Mauri M. Problemas relacionados con los hospederos intermediarios de Fasciola hepatica en Cuba. Serie Biológica 1972;30:1-24.

11. Hernández Silvia, Larramendi Rocío, Mesa J. Observaciones biométricas de Lymnaea cubensis, Pfeiffer 1929 en condiciones naturales. Informe preliminar. Rev Salud Anim 1979;1:25-30.

12. Larramendi Rocío, Hernández Silvia, Mesa J. Lymnaea columella como nuevo hospedero intermediario de Fasciola hepatica en Cuba. Rev Cub Cienc Vet 1981;12:73-76.

13. Boray JC. The potential impact of exotic Lymnaea spp on fascioliasis in Australia. Vet Parasitol 1978;4:127-141.

14. Manga Y, González C, Otero CB. Lymnaea truncatula by the liver fluke Fasciola hepatica in the Proma Basin, León, NW Spain. J Helminthol 1991;65:15-27.

15. Lee CG, Cho SH, Lee SY. Metacercarial production of Lymnaea viridis experimentally infected with Fasciola hepatica. Vet Parasitol 1995;58:313-318.

16. Pfister K, Turner K, Wedrychowics H. Worm recovery, haemagglutinating antibodies and IgE-levels after immunisation against Fasciola hepatica in rats. Vet Parasitol 1980;17:139-150.

17. Facino RM, Carini M, Formo A, Genchi C. Metabolismo hepático del fármaco fasciolicida Nitroxinil en ratas en curso de infestación experimental por Fasciola hepatica. Attidella Soc Ital Cienc Vet 1985;39:794-796.

18. Burden DJ, Ha mmet NC. Fasciola hepatica: attemps to immunise rats using fluke eggs and in vitro culture products. Vet Parasitol 1980;7:51-57.

19. Hughes DL, Harness E, Doy TG. The establishment and duration of Fasciola hepatica infections in two strains of rats and the development of acquired resistance. Res Vet Sci 1976;20:207-211.

20. Mango AM, Mango CKA, Esamal A. Preliminary note on the susceptibility prepatence and recovery of Fasciola gigantica in small laboratory animals. J Helminthol 1972;46:381-386.

21. Goose J, MacGregor M. Naturally acquired immunity to Fasciola hepatica in the rat. Br vet J 1973;129:49-53.

22. Hayes TJ, Mitrovic M. The early expression on protective immunity to Fasciola hepatica in rats. J Parasitol 1977;63:584-587.

23. Goose J. Possible rol of excretory/secretory products in evasion of host defence by Fasciola hepatica. Nature 1978;275:216-217.

24. Kelly JD, Campbell NJ, Dinnen JK. The role of the gut in adquired resistance to Fasciola hepatica in the rat. Vet Parasitol 1980;6:359-367.

25. Thorpe E. Experimental fasciolasis in the albino rat (PHD thesis). Glasgow, Scotland: Veterinary Medicine, University of Glasgow, 1963.

26. Lang BZ. Host-parasitic ralationships of Fasciola hepatica in white mouse. II Studies on required immunity. J Parasitol 1967;53:21-30.

27. Bennett CE, Threadgold LT. Fasciola hepatica: deveploment of the tegument during migration in the white mouse. Exp Parasitol 1975;38:38-56.

28. Rajasekariak JR, Howell MJ. Fasciola hepatica in rats: effects of host age and infective dose. Int J Parasitol 1977;7:119-121.