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2007, Number 1

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Gac Med Mex 2007; 143 (1)

Genómica funcional en embriones y ovocitos humanos individuales

Piña-Aguilar RE

Language: Spanish
References: 8
Page: 89-91
PDF size: 34.88 Kb.


ABSTRACT

During three decades human embryo and oocyte analyses have been performed based on morphological or cytogenetical evaluations; molecular techniques, like FISH and PCR, have been gradually incorporated. However, the development of new techniques of individual cell RNA amplification has allowed the analysis of gene expression in oocytes, cumulus-oocyte complex and preimplantation embryos. These techniques will change radically the study of human reproductive biology and assisted reproduction, allowing a powerful and objective analysis of the complex processes and the interactions that may explain the causes of infertility and generate new therapeutics.


REFERENCES

  1. Adjaye J, Bolton V, Monk M. Developmental expression of specific genes detected in high-quality cDNA libraries from single human preimplantation embryos. Gene 1999;237:373-383.

  2. Neilson L, Andalibi A, Kang D, Coutifaris C, Strauss JF ID, 3 rd, Stanton JA, et al. Molecular phenotype of the human oocyte by PCR-SAGE. Genomics 2000;63:13-24.

  3. Bermudez MG, Wells D, Malter H, Munne S, Cohen J, Steuerwald NM. Expression profiles of individual human oocytes using microarray technology Reprod Biomed Online 2004;8:325-337.

  4. Dobson AT, Raja R, Abeyta MJ, Taylor T, Shen S, Haqq CT, et al. The unique transcriptome through day 3 of human preimplantation development. Hum Mol Genet 2004;13:1461-1470.

  5. 5 Li SST Liu YH, Tseng CN, Singh S. Analysis of gene expression in single human oocytes and preimplantation embryos. Biochem Biophys Res Commun 2006;340:48-53.

  6. Assou S, Anahory T, Pantesco V, Carrour TL, Pellestor F, Klein B, et al. The human cumulus-oocyte complex gene-expression profile. Hum Reprod 2006;21:1705-1719.

  7. Kocabas AM, Crosby J, Ross PJ, Otu HH, Beyhan Z, Can H, et al. The transcriptome of human oocytes. Proc Natl Acad Sci USA 2006;103:1427-1432.

  8. Piña-Aguilar RE, López-Saucedo J, Dumonteil E. Contribución del espermatozoide al transcriptoma del embrión humano preimplantación. Memorias ID Congreso Regional de Biotecnología y Bioingeniería del Sureste. 5MBB.Mérida, Yucatán 20-22 Sep, 2006.




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