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1993, Number 2

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Vet Mex 1993; 24 (2)

Determination of hyaluronic acid presence in the capsular material of Pasteurella multocida types A and D isolated from pig lungs

Gutierrez PJA, Smith JE

Language: English/Spanish
References: 22
Page: 113-116
PDF size: 807.59 Kb.


ABSTRACT

A total of 20 strains of P. multocida was isolated frorn pig lungs. Seven smooth colony strains belonged to serotype D, whereas 12 highly mucoid and one smooth strains were classified as type. A by the indirect haemag-glutination test. A saIt gradient paper chromatography was used for the detection of hyaluronic acid. All P. multocida strains tested, developed a blue spot at the same level as the purified hyaluronic acid did. Serotype A strains showed a more distinct and bigger spot than serotype D cultures. Findings suggest that the capsular material of serotype D strains may contain hyaluronic acid as serotype A does, or may have a different substance that migrates to the same Ievel.


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