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2007, Number 4

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Rev Inst Nal Enf Resp Mex 2007; 20 (4)

Agreement of the systemic arterial pressure in normotensive rats, determined by a non-invasive method

Flores CPL, Santos MLE, Martínez MR, Cortés TS, Sánchez TG, Infante VO

Language: Spanish
References: 18
Page: 247-254
PDF size: 99.86 Kb.


ABSTRACT

During the validation process, repeatability by gold standard methods is very important. Previously, we have tested a non-invasive method to determine the systemic arterial pressure in rats; unlike others, this method is new, feasible and of low cost. In this work we evaluated the degree of agreement between the arterial pressure determined by the invasive way and with our non-invasive method.
Methods: A concordant design was undertaken to study 10 three month old normotensive, 250 to 300 g bodyweight Wistar rats. Under sedation, the arterial pressure was determined simultaneously by both methods.
Results: The systolic arterial pressures obtained with invasive vs. non-invasive methods respectively were similar: 111.43 ± 14.94 vs. 112.73 ± 15.19, p ‹ 0.467, and diastolic 84.94 ± 14.44 vs. 88.35 ± 15.67, p ± 0.272. Intraclass correlation coefficient for the systolic arterial pressure was 0.966 (0.86, 0.99) and diastolic 0.898 (0.585, 0.974). Mean differences ± standard deviation and its respective limits of agreement for systolic arterial pressure were -1.3 ± 5.4 (+9.5, -12.1) and diastolic -3.41 ± 9.20 (+14.99, -21.81).
Conclusion: This non-invasive method reproduces the systemic arterial pressure in a reliablnormotensive rats. We study the possibilities to modify our design and apply it to the pulmonary circuit.


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