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Anales de Otorrinolaringología Mexicana

2006, Number 3

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Otorrinolaringología 2006; 51 (3)

Intracerebral injection (sustantia nigra compacta) of dextran ferrum as an experimental model of Parkinson’s disease

Alfaro RA, González PR, Padilla MK, Uribe ER

Language: Spanish
References: 27
Page: 105-110
PDF size: 280.12 Kb.


ABSTRACT

Background
Parkinson’s disease (PD) is featured by progressive and selective destruction in the nigrostriatal dopaminergic neurons. Dopamine’s oxidative metabolism, the hydrogen peroxide (H2O2) generation and the production of the 6-hydroxydopamine neurotoxin have been associated. H2O2 may damage directly to the tissues or facilitate the productions of oxygen free radicals. Such a fact is enhanced by low levels of antioxidant enzymes such as glutation peroxidase, superoxido dismutase and catalase, which are found in the central nervous system in some extent.

Objective
To develop a Parkinson’s disease experimental model by means of the study of the neurochemical and behavioral effects after the administration of 1μl of dextran Ferrum (D Fe) into the sustantia nigra compacta (SNc) by means of a micropipette (10 μm thick).

Material and methods
40 male wistar rats were used (260-310 g). D Fe was injected in the right SNc and their motor performance was evaluated during 30 days. Another group was decapitated after either, 7 or 30 days in order to obtain the striatum dopamine content were analyzed by means of high performance liquid chromatography. Results were analyzed by means of an ANOVA and a pos hoc Tukey t test (p ‹ 0.001).

Results
Behavioral test showed that the animals maintained a head’s inclination contralateral to the microinjection’s site, increasing gradually along time post-injection. Moreover, contralateral fore and hindlimb crawling, and contralateral rotational movements were also observed. On the other hand, dopamine levels decreased after 7 and 30 days post-injection.

Conclusion
The D Fe injection accelerated the peroxydation mechanisms, leading to a progressive development of Parkinson's disease.


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