medigraphic.com
ISSN 2992-8036 (Electronic)
ISSN 2306-4102 (Print)
Órgano Oficial del Colegio Mexicano de Ortopedia y Traumatología

2007, Number 4

<< Back Next >>

Acta Ortop Mex 2007; 21 (4)

Results of the experimental repair of osteochondral lesions in a pig model using tissue engineering

Villalobos CFE, Velasquillo MC, Martínez LV, Lecona BH, Reyes MB, Estrada VE, Villegas CH, Solís AL, Espinosa MR, Ibarra PLC

Language: Spanish
References: 21
Page: 217-223
PDF size: 420.73 Kb.


ABSTRACT

Objective: To repair experimental osteochondral knee lesions in pigs using tissue engineering. Material and methods: Eight 40-kg pigs underwent surgery. Cartilage and periosteal biopsies of their control knee were taken. Cartilage and periosteal cells were independently isolated, cultured and seeded in biodegradable PGA and PLA polymers that were fixed on the bottom of an osteochondral defect in the pig’s experimental knee, with bioabsorbable Mitek implants. Four months later the pigs were sacrificed and the knees were analyzed with nuclear magnetic resonance imaging (NMRI), macroscopic assessment, histology, electron microscopy (EM), scanning electron microscopy (SEM) and SEM element analysis. Results: All the defects were filled with cartilage-like tissue according to the NMRI evaluation and the visual examination. Hyaline-like cartilage was obtained in 3 defects and fibrocartilage in 5. The EM showed chondrocytes in the repair tissue. The SEM showed appropriate integration to the bone and the surrounding tissue. SEM element analysis showed sulphurized matrix attached to the bone with calcium and phosphates as predominant elements. Discussion: Tissue engineering enabled the production of tissues similar to normal ones. The polymer fixation system was effective.


REFERENCES

  1. Farmer JM, Boles CA: Chondral and osteochondral injuries. Diagnosis and management. Clin Sports Med 2001; 20(2): 299-319.

  2. Maiotti M, Coletta M: Correlation between osteoarthritic cartilage damage and levels of proteinases and proteinase inhibitors in synovial fluid from the knee joint. Arthroscopy 2000; 16(5): 522-6.

  3. Elders MJ: The increasing impact of arthritis on public health. J Rheumatol Suppl, 2000; 60: 6-8.

  4. Villalobos CE, Almazán DA, Cruz LF, Encalada DI, Pérez JF, Izaguirre HA, Ibarra PC: Lesiones del cartílago articular en 1,309 artroscopías de rodilla. Acta Ortopédica Mexicana, (en prensa).

  5. Cole BJ, Malek MM: Articular cartilage lesions – A practical guide to assessment and treatment. Ed. Springer-Verlag. China, 2004.

  6. Sandell LJ, Grodzinsky AJ: Tissue engineering in musculoskeletal clinical practice. Ed. American Academy of Orthopaedic Surgeons. Estados Unidos de América, 2003.

  7. Freed LE, Langer R: Cultivation of cell-polymer cartilage implants in bioreactors. J Cell Biochem 1993; 51(3): 257-64.

  8. Freed, LE, Vunjak-Novakovic G: Frontiers in tissue engineering. In vitro modulation of chondrogenesis. Clin Orthop 1999; (367 Suppl): S46-58.

  9. Cao Y, Rodriguez A, Vacanti M, Ibarra C, Arevalo C, Vacanti CA: Comparative study of the use of polyglycolic acid, calcium alginate and pluronics in the engineering of autologous porcine cartilage. J Biomater Sci Polym Ed, 1998; 9(5): 475-87.

  10. Ibarra C, Janneta C, Vacanti CA, Cao Y, Kim TH, Upton J, Vacanti JP: Tissue engineered meniscus: a potential new alternative to allogeneic meniscus transplantation. Transplant Proc 1997; 29(1-2): 986-8.

  11. Koski JA, Ibarra C, Rodeo SA, Warren RF: Meniscal injury and repair: clinical status. Orthop Clin North Am 2000; 31(3): 419-36.

  12. Rodriguez A, Cao YL, Ibarra C, Pap S, Vacanti M, Eavey RD, Vacanti CA: Characteristics of cartilage engineered from human pediatric auricular cartilage. Plast Reconstr Surg 1999; 103(4): 1111-9.

  13. Cao Y, Vacanti CA: Transplantation of chondrocytes utilizing a polymer-cell construct to produce tissue-engineered cartilage in the shape of a human ear. Plast Reconstr Surg 1997; 100(2): 297-302.

  14. Hidaka C, Ibarra C, Hannafin J, Torzilli P, Quitoriano M, Jen SS, Warren R, Crystal R: Formation of vascularized meniscal tissue by combining gene therapy with tissue engineering. Tissue Engineering 2002; 8 (1): 93-105.

  15. Caplan A: Principles of tissue engineered regeneration of skeletal tissues. Clinical Orthopaedics and Related Research 1999; 367S: 12-16.

  16. Roberts S, Richardson JB: Autologous chondrocyte implantation for cartilage repair: monitoring its success by magnetic resonance imaging and histology. Arthritis Res Ther 2003; 5(1):60-73.

  17. Peterson L, Minas T, Brittberg M, Lindahl A: Two-to 9-year outcome after autologous chondrocyte transplantation of the knee. Clinical Orthopaedics and Related Research 2000; 374: 212-34.

  18. Brittberg M: Articular cartilage engineering with autologous chondrocyte transplantation–A review of recent development. J Bone Joint Surg 2003; 85-A: 109-15.

  19. Chiang H, Jiang Ch: Repair of porcine articular cartilage defect with autologous chondrocyte transplantation. Journal of Orthopaedic Research 2005; 23: 584-93.

  20. Rudert M, Wirth CJ: Cell-based treatment of osteochondral defects in the rabbit knee with natural and synthetic matrices: cellular seeding determines the outcome. Arch Orthop Trauma Surg 2005; 2: 1-11.

  21. Schek RM, Krebsbach PH: Engineered ostechondral grafts using biphasic composite solid free-form fabricated scaffolds. Tissue Eng 2004; (9-10): 1376-85.




2020     |     www.medigraphic.com