Evelson P, Susemihl C, Villarreal I, Llesuy S, Rodríguez R, Peredo H, Lemberg A, Perazzo J, Filinger E

Language: English
References: 36
Page: 115-120
PDF size: 76.95 Kb.

Text Extraction

Oxidative stress (OS) is a biological entity quoted as responsible for several pathologies including diabetes. Diabetes mellitus (DM) has been also associated to human cirrhosis. The present work was designed to study the occurrence of OS as well as morphologic alterations in rat livers following induction of DM. Two groups of rats were used: Control and Diabetic. DM was induced in the second group by streptozotocin (STZ) in a single dose of 60 mg/kg, injected i.p. The occurrence of OS was determined in liver homogenates by measuring the hydroperoxide-initiated chemiluminescence and the activity of antioxidant enzymes (superoxide dismutase, catalase and glutathione peroxidase). Liver sinusoids were morphometrically analyzed. In conclusion, livers from the diabetic group did not show evidence of the occurrence of OS, as it would be expected, but dilation of hepatic sinusoids was documented and it was significantly different from control group.

REFERENCES

1. Rodriguez R, Poucheret P, Battell M, McNeill H. In: Experimental models of diabetes. J. McNeill ed. CRC Press. 1999: 3-17.

2. Veelken R, Hilgers K, Hartner A, Haas A, Bohnier K, Sterzel R. Nitric oxide synthase isoforms and glomerular hyperfiltration in early diabetic nephropathy. J Am Soc Nephrol 2000; 11:71-9.

3. Alder VA, Su E, Yu D, Cringle S, Yu PK. Diabetic retinopathy: early functional changes. Clin Exp Pharmacol Physiol 1997; 10: 785-88.

4. Cairus SR, Peters TJ. Biochemical analysis of hepatic lipid in alcoholic and diabetic and control subjects. Clin Sci 1983; 65: 645-52.

5. Glaseer S, Selwyn A, Ganz P. Atherosclerosis: risk factors and the vascular endothelium. Am Heart J 1996; 131: 379-84.

6. Kakkar R, Kalza J, Mantha S, Prasad K. Lipid peroxidation and activity of antioxidant enzymes in diabetic rats. Mol Cell Biochem 1995; 151: 113-9.

7. Matkovics B, Kotorman M, Varga S, Hai DQ, Varga C. Oxidative stress in experimental diabetes induced by streptozotocin. Acta Physiol Hung 1997; 85: 29-38.

8. Wolff SP, Dean RT. Glucose autoxidation and protein modification: The role of oxidative glycosylation in diabetes. Biochem J 1987; 245: 234-50.

9. Tomlinson KC, Gardiner SM, Hebden RA, Bennett T. Functional consequences of STZ-induced DM, with particular reference to the CV system. Pharmacol Reviews 1992; 41: 104-31.

10. Raza H, Prabu SK, Robin MS, Avadhani NG. Elevated mitocondrial cytochrome P450 2E1 and glutathione S-transferase A4-4 in STZ-induced diabetic rats: tissue-specific variations and roles in oxidative stress. Diabetes 2004; 53: 185-94.

11. Otsyula M, King MS, Ketcham TG, Sanders RA, Watkins JB 3rd.. Oxidative stress in rats after 60 days of hypergalactosemia or hyperglycemia. Int Toxicol 2003; 22: 423-27.

12. Anwar MM, Meki AR. Oxidative stress in STZ-induced diabetic rats: effects of garlic oil and melatonin. Comp Biochem Physiol A Mol Integr Physiol 2003; 135: 539-47.

13. Genet S, Kale RK, Baquer NZ. Alterations in antioxidant enzymes and oxidative damage in experimental diabetic rat tissues: effect of vanadate and fenugreek (Trigonellafoenum graecum). Mol Cell Biochem 2002; 236: 7-12.

14. Llesuy S, Evelson P, González Flecha P, Peralta J, Carreras M, Poderoso J, Boveris A. Oxidative stress in muscle and liver of rats with septic syndrome. Free Radic Biol & Med 1994; 16: 445-51.

15. González Flecha B, Llesuy S, Boveris A. Hydroperoxide-initiated chemiluminiscence: an assay for oxidative stress in biopsies of heart, liver and muscle. Free Radic Biol & Med 1991; 10: 93-100.

16. Misra, I. Fridovich, I. The generation of superoxide radical during the autoxidation of ferredoxins. J Biol Chem 1971; 246: 6886-90.

17. Chance B. In: Special methods: catalase. R. Glick. ed. Interscience. New York. 1954: 408-24.

18. Flohé L, Gunzler A. Assays of Glutatione Peroxidase. Methods Enzymology 1984: 114-21.

19. Lowry OH, Rosembrough NJ, Farr AL, Randall, RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951; 193: 265-75.

20. Miyai K. Structural organization of the liver. In: Neeks RG, Harrison SD, Bull RJ, eds. Hepatotoxicology, Boca Ratón: CRC Press, 1991: 1-65.

21. Ling X, Nagai R, Sakashita N, Takeya M, Horiuchi S, Takahashi K. Immunohistochemical distribution and quantitative biochemical detection of advanced glycation end products in fetal to adult rats and in rats with streptozotocin-induced diabetes. Lab Invest 2001; 81: 845-61.

22. Otterbein LE, Choi AM. Heme oxygenase: colours of defence against cellular stress. Am J Physiol 2000; 279: L1029-37.

23. Otterbein LE, Soares MP, Yamashita K, Bach FH. Heme oxygenase-1: unleashing the protective properties of heme. Trends Inmunol 2003; 24: 449-55.

24. Boveris A, Fraga C, Varsavsky A, Koch O. Increased chemiluminescence and superoxide production in the liver of chronically ethanol-treated rats. Arch. Biochem. Biophys 1983; 227: 534-541.

25. Fraga C, Llesuy S, Boveris A. Increased carbon tetrachloride-stimulated chemiluminescence in the in situ liver of barbital treated mice. Acta Physiol Pharmacol. Latinoamer 1984; 34: 143-150.

26. Boveris A, Llesuy S, Fraga C. Increased liver chemiluminescence in tumor-bearing mice. J Free Radic Biol & Med 1985; 1: 131-138.

27. Milei J, Boveris A, Llesuy S, Molina H, Storino R, Ortega D, Milei S. Amelioration of adriamycin induced cardiotoxicity in rabbits by prenilamine and vitamins A and E. Am Heart J 1986; 111: 95-102.

28. Llesuy S, Milei J, González Flecha B, Boveris A. Myocardial damage induced by doxorubicins: hydroperoxide-initiated chemiluminescence and morphology. J Free Radic Biol & Med 1990; 8: 259-264.

29. Ramanathan M, Jaiswal A, Bhattacharya. Superoxide dismutase, catalase and glutathione peroxidase activities in the brain of streptozotocin induced diabetic rats. Indian J Exp Biol 1999; 37: 182-3.

30. Karasu C. Increased activity of H2O2 in aorta isolated from chronically streptozotocin-diabetic rats: effects of antioxidant enzymes and enzymes inhibitors. Free Radic Biol & Med 1999; 27: 16-27.

31. Moore S, Bohlen H, Miller B, Evan A. Cellular and vessel wall morphology of cerebral cortical arterioles after short-term diabetes in adult rats. Blood Vessels 1985; 22: 265-77.

32. Murata T, Nakagawa K, Klialil A, Ishibashi T, Inornata H, Sueishi K. The relation between expression of vascular endothelial growth factor and breakdown of the blood-retinal barrier in diabetic rat retinas. Lab Invest 1996; 74: 819-25.

33. Bazan N, Gordon W, Marcheselli V, Lukiw W, Duhault J, Koenig-Berard E, Linn D, et al. Experimental models and their use in studies of diabetic retinal microangiopathy. Therapie 1997; 52: 447-51.

34. Bassirat M, Khalil Z. Endothelin and free radicals modulate microvascular responses in streptozotocin-induced diabetic rats. Microvasc Res 2000; 59: 88-98.

35. Turk J, Corbett J, Ramanadham S, Bohrer A, McDaniel M. Biochemical evidence for nitric oxide formation from streptozotocin in isolated pancreatic islets. Biochem Biophys Res Commun 1993; 197: 1458-64.

36. Huszka M, Kaplar M, Rejto L, Tomai I, Palatka K, Laszlo P, Udvardy M. The association of reduced endothelium derived relaxing factor-NO production with endothelial damage and increased in vivo platelet activation in patients with diabetes mellitus. Thromb Res 1997; 86: 173-80.