Ruiz RRA, Candanosa AE, Sánchez GF, Ducoing WA

Language: English/Spanish
References: 28
Page: 41-53
PDF size: 496.50 Kb.

ABSTRACT

Thirty cases of small intestine with suggestive histopathological lesions of canine parvovirus were evaluated by avidin-biotinperoxidase complex using a monoclonal antibody developed in mouse against canine parvovirus-2 (CPV-2). Positive immunohistochemical reactions were obtained in 76.67% (23 cases) of processed samples. The Iymphocytes, macrophages and necrotic cells of the intestinal crypts were the cells that most frequently showed immunopositivity. The intestinal histopathological lesions which were associated to CPV-2 were the regeneration cells in the intestinal crypts by the evidence of parvoviral antigen. The immunohistochemical demonstration of parvoviral antigen is a useful and reliable method for confi rming an etiologic diagnosis in cases with suggestive intestinal lesions of this disease.

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